ZFIN ID: ZDB-FIG-150330-32
Fukui et al., 2014 - S1P-Yap1 Signaling Regulates Endoderm Formation Required for Cardiac Precursor Cell Migration in Zebrafish. Developmental Cell   31:128-136 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: 5-9 somites to 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 14-19 somites

Fig. 3

Yap1 Regulates Endodermal Cell Survival

(A) Time-sequential (from eight-somite stage [8s] to 16-somite stage [16s]) images of Tg(sox17:GFP) embryos injected with MO indicated at the left. Arrows denote the fragmentation of sox17 promoter-driven GFP-positive cells. A set of representative images of eight independent experiments is shown. Dorsal view.

(B) Incidence of cardia bifia (black) and normal heart (white) in the morphants exhibiting less than 5 fragmented endodermal cells or more than 5 fragmented endodermal cells in the embryos treated with the MO indicated at the bottom.

(C) Bar graph shows the number of the TUNEL-positive cells among the GFP-positive endodermal cells of Tg(sox17:GFP) embryos at 14-somite stage (14s) injected with MO indicated at the bottom. ctrl MO, control morpholino. p < 0.01 (versus ctrl morphants).

(D) Bar graph shows the number of the TUNEL-positive cells in a S1pr2 mutant (mil), heterozygous embryo (s1pr2m93/+) and homozygous embryos (s1pr2m93/m93). p < 0.01.

(E) Gene expression profile analyzed by RNA-seq using RNAs obtained from the Tg(sox17:GFP) embryos uninjected (control) and injected with yap1 MO by sorting of GFP-positive endodermal cells. The graph shows the ratio of the reads per kilobase per million reads (RPKM) of the yap1 morphants-derived RNAs divided by that of the control. Among the genes we analyzed, those regulating cell survival are listed.

See also Figure S3 and Movies S1, S2, and S3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ha01Tg control 5-9 somites endoderm IFL
10-13 somites endoderm IFL
14-19 somites endoderm IFL
ha01Tg + MO1-yap1 standard conditions 5-9 somites endoderm IFL
10-13 somites endoderm IFL
14-19 somites endoderm IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ha01Tg + MO1-s1pr2 standard conditions 14-19 somites cardiac muscle progenitor cell migration to the midline involved in heart field formation decreased occurrence, abnormal
14-19 somites endoderm anterior region apoptotic, abnormal
14-19 somites endoderm apoptotic process increased occurrence, abnormal
14-19 somites heart morphogenesis decreased process quality, abnormal
14-19 somites heart tube split bilaterally, abnormal
ha01Tg + MO1-yap1 standard conditions 14-19 somites cardiac muscle progenitor cell migration to the midline involved in heart field formation decreased occurrence, abnormal
14-19 somites endoderm anterior region apoptotic, abnormal
14-19 somites endoderm apoptotic process increased occurrence, abnormal
14-19 somites heart morphogenesis decreased process quality, abnormal
14-19 somites heart tube split bilaterally, abnormal
ha01Tg + MO3-ccn2a standard conditions 14-19 somites cardiac muscle progenitor cell migration to the midline involved in heart field formation decreased occurrence, abnormal
14-19 somites endoderm anterior region apoptotic, abnormal
14-19 somites endoderm apoptotic process increased occurrence, abnormal
14-19 somites heart morphogenesis decreased process quality, abnormal
14-19 somites heart tube split bilaterally, abnormal
s1pr2m93/m93 standard conditions 14-19 somites cardiac muscle progenitor cell migration to the midline involved in heart field formation decreased occurrence, abnormal
14-19 somites endoderm anterior region apoptotic, abnormal
14-19 somites endoderm apoptotic process increased occurrence, abnormal
14-19 somites heart morphogenesis decreased process quality, abnormal
14-19 somites heart tube split bilaterally, abnormal
Acknowledgments:
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Reprinted from Developmental Cell, 31, Fukui, H., Terai, K., Nakajima, H., Chiba, A., Fukuhara, S., Mochizuki, N., S1P-Yap1 Signaling Regulates Endoderm Formation Required for Cardiac Precursor Cell Migration in Zebrafish, 128-136, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell