ZFIN ID: ZDB-FIG-150120-4
Smith et al., 2014 - Contact-Mediated Inhibition Between Oligodendrocyte Progenitor Cells and Motor Exit Point Glia Establishes the Spinal Cord Transition Zone. PLoS Biology   12:e1001961 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec

Fig. 4

sox10+ motor root glia arise from olig2+ precursors in the spinal cord.

(A) Frames captured from a 24-h time-lapse video of a Tg(olig2:dsred) larvae showing olig2+ cells (arrow) migrating out of the CNS, pinching at the MEP, and associating with the spinal motor root axons. Numbers in lower right corners denote time lapsed from the first frame of the figure. Brackets show the CNS and PNS portions in the images. (B) In Tg(sox10:eos) embryos injected with a nkx2.2a MO, CNS-derived sox10+ motor glial cells (arrow) are indistinguishable from wild-type nerves. In contrast, in olig2 MO-injected embryos, motor root glial cells are absent. In both instances, sensory glial cells are unaffected (arrowheads). (C) Quantification of data from panel B (>50 nerves were scored for each perturbation). (D) In a Tg(sox10:eos);Tg(olig2:dsred) embryo at 56 hpf, a sox10+ cell located on the motor nerve root expresses olig2 and sox10 (open arrowhead). Neighboring sox10+ cells in the DRG (asterisk) do not express olig2. (E) In a Tg(nkx2.2a:megfp);Tg(olig2:dsred) embryo at 72 hpf, nkx2.2+ perineurial glia are distinct from olig2+ MEP glia (open arrowhead). All images are lateral views of the motor and sensory root with dorsal to the top and anterior to the left. Scale bars, 25 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 vu17Tg; vu19Tg chemical treatment: N-phenylthiourea Long-pec glial cell IFL
vu19Tg chemical treatment: N-phenylthiourea Long-pec primary motor neuron IFL
vu19Tg; w9Tg chemical treatment: N-phenylthiourea Long-pec primary motor neuron IFL
EGFP vu17Tg; vu19Tg chemical treatment: N-phenylthiourea Long-pec glial cell IFL
Eos vu19Tg; w9Tg chemical treatment: N-phenylthiourea Long-pec glial cell IFL
w9Tg + MO4-nkx2.2a ultraviolet light, chemical treatment: N-phenylthiourea Long-pec glial cell IFL
w9Tg + MO4-olig2 ultraviolet light, chemical treatment: N-phenylthiourea Long-pec glial cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.