FIGURE

Fig. S2

ID
ZDB-FIG-140624-9
Publication
O'Brien et al., 2014 - MicroRNA-30a regulates zebrafish myogenesis through targeting the transcription factor Six1
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Fig. S2

Morpholino studies reveal that six1a and six1b function together during zebrafish myogenesis. Live images (A) at 24 hours post fertilization (hpf), anterior to the left, show that lower doses of six1a MO and six1b MO injected alone do not disrupt normal somite morphology individually, only when injected together are U-shaped somites observed (control: n=75/77, 6.5 ng six1a MO: n=61/64, 2.5 ng six1b MO: n=50/56, six1a/b MO: n=100/154). Lateral views of in situ hybridization (B) at 9 somites shows an early delay of tropomyosin-α (tpma) (control: n=25/27, six1a MO: n=21/25, six1b MO: n=20/25, six1a/b MO: n=10/14), which is strongest in the double six1a/b knockdown. Scalebar = 100 µm.

Expression Data
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 5-9 somites

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.