FIGURE

Fig. 6

ID

ZDB-FIG-140624-24

Publication

Samson et al., 2013 - 3-OST-7 regulates BMP-dependent cardiac contraction

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Fig. 6

3-OST-7 regulates cardiac contraction by constraining BMP signaling.

(A) Tg(hs:bmp2b) heterozygotes were crossed to wild-type zebrafish and embryos were either untreated (no hs) or heat-shocked at 12 hpf. Embryos in each group were scored for ventricular contraction, and then genotyped for presence of heat-shock transgene. Graph depicts percentage contraction of embryos with transgene (green) or without (blue) in each treatment group. Induction of BMP signaling led to ventricular noncontraction. (B) bmp4^st72 heterozygotes were crossed and embryos were either uninjected or injected with 3-OST-7 MO. Embryos in each group were scored for ventricular noncontraction, and then genotyped for bmp4 mutation (RE, digestion with SpeI). Graph depicts percentage contraction of each genotypic class in uninjected embryos (blue) or embryos injected with 3-OST-7 MO (red). Ventricular noncontraction was rescued in 3-OST-7 morphants by bmp4^st72 mutation.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-hs3st1l1 WT + MO2-hs3st1l1 fr13Tg/+
Condition:	heat shock
Knockdown Reagents:	MO1-hs3st1l1 MO2-hs3st1l1
Observed In:	cardiac ventricle heart contraction
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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