ZFIN ID: ZDB-FIG-140523-57
Chen et al., 2014 - The development of zebrafish tendon and ligament progenitors. Development (Cambridge, England)   141:2035-45 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth

Fig. 5 The role of muscle in the specification and maintenance of tendon populations. (A,B) Loss of myod1 and myf5 results in the complete absence of myog-positive differentiated muscles in the head (left), fin (middle) and tail (right) (93%, n=67). (C-F) In myod1-myf5-deficient embryos at 53-58hpf, scxa expression is lost in the myosepta (C,D), and xirp2a expression is completely absent in the craniofacial, pectoral fin and myoseptal tissue (E,F) compared with controls. However, loss of differentiated muscle (C,D) does not alter expression of scxa-positive tendon progenitors in the craniofacial or pectoral fin tissue (97%, n=32). (G-N) At 72hpf, myod1–/– and myf5-deficient embryos have (G,H,K,L) complete loss of myosin heavy chain (MHC) expression in the craniofacial and pectoral fin tissue and (I,J) a virtual loss of scxa expression in the head and pectoral fin tissue. (M,N) Expression of xirp2a is also missing (100%, n=19). Fluorescent images of MHC-stained flat-mounted embryos in G,H,K,L correspond to the same embryos in brightfield (I,J,M,N).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myog WT standard conditions Long-pec head muscle ISH
Long-pec pectoral fin musculature ISH
Long-pec trunk musculature ISH
WT + MO1-myf5 + MO1-myod1 standard conditions Long-pec Not Detected head muscle ISH
Long-pec Not Detected pectoral fin musculature ISH
Long-pec Not Detected trunk musculature ISH
scxa WT standard conditions Long-pec myoseptum ISH
Long-pec pectoral fin tendon ISH
Long-pec pharyngeal arch tendon ISH
Protruding-mouth pectoral fin tendon ISH
Protruding-mouth pharyngeal arch tendon ISH
WT + MO1-myf5 + MO1-myod1 standard conditions Long-pec Not Detected myoseptum ISH
Long-pec pectoral fin tendon ISH
Long-pec pharyngeal arch tendon ISH
myod1fh261/fh261 + MO1-myf5 standard conditions Protruding-mouth Not Detected pectoral fin tendon ISH
Protruding-mouth Not Detected pharyngeal arch tendon ISH
xirp2a WT standard conditions Long-pec myoseptum ISH
Long-pec pectoral fin ISH
Long-pec pharyngeal arch ISH
Protruding-mouth pectoral fin ISH
Protruding-mouth pharyngeal arch ISH
WT + MO1-myf5 + MO1-myod1 standard conditions Long-pec Not Detected myoseptum ISH
Long-pec Not Detected pectoral fin ISH
Long-pec Not Detected pharyngeal arch ISH
myod1fh261/fh261 + MO1-myf5 standard conditions Protruding-mouth Not Detected pectoral fin ISH
Protruding-mouth Not Detected pharyngeal arch ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-A4.1025 IHC WT standard conditions Protruding-mouth pectoral fin musculature
IHC Protruding-mouth pharyngeal musculature
IHC myod1fh261/fh261 + MO1-myf5 standard conditions Protruding-mouth Not Detected pectoral fin musculature
IHC Protruding-mouth Not Detected pharyngeal musculature
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development