FIGURE

Fig. 6

ID
ZDB-FIG-140523-58
Publication
Chen et al., 2014 - The development of zebrafish tendon and ligament progenitors
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Fig. 6

The role of cartilage in the specification and organization of tendon and ligaments. col2a1-positive pharyngeal cartilage was lost in sox9a- (B) and sox9a-sox9b-deficient embryos (88%, n=56) (C) compared with controls (A). (D-F) sox9a- and sox9a-sox9b-deficient embryos (93%, n=42) express scxa but in an altered pattern compared with controls. sox9a-sox9b-deficient embryos were identified by the loss of their otic vesicle, which only occurs in the absence of both genes. Similar expression was observed for morpholino-mediated knockdown of sox9a and sox9b together, and with sox9a or sox9b morpholino injection into crosses of sox9ahi1134 or sox9bfh313 mutants, respectively. (G,H,J,K) At 72hpf and 96hpf, sox9a- and sox9a-sox9b-deficient embryos (82%, n=57) express scxa and tnmd, but in an abnormal pattern compared with controls. The scxa- and tnmd-expressing ligaments (H,I,K,L, arrows) appear shorter and wider than controls. Expression posterior and lateral to the ligaments in K,L marks the branchiostegal rays.

Expression Data
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Long-pec to Day 4

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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