FIGURE

Fig. S4

ID

ZDB-FIG-140211-22

Publication

Dyer et al., 2014 - A bi-modal function of Wnt signalling directs an FGF activity gradient to spatially regulate neuronal differentiation in the midbrain

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Fig. S4

Wnt and FGF signalling regulate each others activity through feedback loops. Dorsal views of embryos processed by in situ hybridisation with probes to dgfp (A-E), fgf8a (F-I), pea3 (J-M), wnt1 (N-Q), lef1 (R-U) in 24 hpf embryos from Tg[top:dgfp] (A-E) or Tg[UAS:HA-bcat]; Tg[hsp70:gal4] (F-U) transgenics. Embryos were treated with 10μM SU5402 (H,I,L,M,P,Q,T,U) or DMSO (F,G,J,K,N,O,R,S) from 14 hpf, then heat-shocked at 16.5 hpf; embryos over-expressing betacatenin were sorted based on HA expression. Scale bars 100μm (A-U).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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