FIGURE

Fig. 5

ID

ZDB-FIG-140204-50

Publication

Westhoff et al., 2013 - Development of an Automated Imaging Pipeline for the Analysis of the Zebrafish Larval Kidney

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Fig. 5

Detection of cystic kidneys after Ift80 and Ift172 knockdown.

(A) Automatically generated overview thumbnail image of 96 kidneys of morpholino injected larvae: standard ctr-MO (row 1-2), ift80-MO (row 3-5) and ift172-MO (row 6-8). (B-D) Phenotypic alterations of zebrafish larvae at 4 dpf after morpholino microinjection; (C) ift80-MO and (D) ift172-MO injected embryos exhibit glomerular cyst (arrow) and a ventrally curved tail compared to (B) standard ctr-MO injected embryos. (E-G) In-detail visualisation of glomerular cysts at 72 hpf in (F) ift80-MO and (G) ift172-MO injected embryos compared to standard ctr-MO injected embryos (E) using the Tg(wt1b:EGFP) transgenic line.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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