Fig. 4

Localization of cyclin B1 reporter mRNAs in the animal polar cytoplasm of transgenic zebrafish oocytes. (A) Section in situ hybridization of a full-grown oocyte probed with cyclin B1. The cyclin B1 mRNAs were localized to the animal polar cytoplasm of the oocyte beneath the micro-pile as an aggregation (arrow). ((B)–(H)) Section in situ hybridization probed with gfp, showing full-grown oocytes of transgenic zebrafish expressing tgo3′ (B), 1–523 (C), 524–1197 (D), 524–736 (E), 737–949 (F), 949–1197 (G) and tgo^M3′ (H) mRNAs. The tgo3′, 524–1197 and 524–736 mRNAs were aggregated beneath the micro-pile (arrows) ((B), (D), (E)), whereas the 1–523, 737–949, 949–1197 and tgo^M3′ mRNAs had no signal ((C), (F), (G), (H)). Arrowheads indicate the micro-pile. Dotted lines encircle aggregated mRNAs. Bars, 100 μm.