FIGURE

Fig. 5

ID

ZDB-FIG-131108-18

Publication

Choorapoikayil et al., 2013 - Loss of Pten promotes angiogenesis and enhanced vegfaa expression in zebrafish

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Fig. 5

Combined treatment with LY294002 and sunitinib rescued hypervascularization. (A) Quantitative PCR was performed to determine vegfaa expression at 4 dpf in ptena^-/-ptenb^-/- mutants compared with siblings following treatment with suboptimal concentrations of LY294002 (LY, 5 μM) or sunitinib (5 μM), or both, from 72 hpf onwards. Three embryos per condition were pooled and used for quantitative PCR; at least two independent experiments were performed. Relative amounts were determined with wild type untreated set to 1.0. Statistical analysis (Kruskal-Wallis with Dunn’s post-hoc test) was performed using Excel and SPSS 20 (IBM); significance is indicated: **P<0.01; ***P<0.001. (B–I) Vasculature of wild-type and ptena^-/-ptenb^-/- embryos at 4 dpf was imaged in the Tg(kdrl:eGFP) line by confocal microscope with 20×. The embryos were treated with suboptimal concentrations of LY294002 (LY), sunitinib or both as indicated. Representative embryos are depicted; anterior to the left.

Expression Data

Gene:	EGFP
Fish:	s843Tg ptena^{hu1864/hu1864}; ptenb^{hu1435/hu1435}; s843Tg
Condition:	chemical treatment: pharmaceutical
Anatomical Term:	trunk vasculature
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ptena^{hu1864/hu1864}; ptenb^{hu1435/hu1435}; s843Tg
Observed In:	angiogenesis blood vessel trunk vasculature
Stage:	Day 4

Phenotype Detail

Acknowledgments

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