FIGURE

Fig. 2

ID

ZDB-FIG-130211-3

Publication

Nair et al., 2013 - In vitro oocyte culture-based manipulation of zebrafish maternal genes

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Wild-type aurB mRNA injected into stage IV cei/aurB oocytes rescues the maternal-effect phenotype in cei/aurB embryos. A–L: Animal views of 65 mpf blastodiscs immunolabeled to detect α-tubulin (A,E,I) and β-catenin (B,F,J), with DAPI (4′,6-diamidine-2-phenylidole-dihydrochloride) stainings (C,G,K) and panel merges (D,H,L). A,B,E,F: Wild-type embryos show robust furrows between cells and β-catenin accumulation at the furrows (A,B), while cei/aurB embryo from uninjected cei/aurB stage IV oocyte shows partial, rudimentary furrows (E), which do not accumulate β-catenin (F). I,J: Rescued cei/aurB embryo from cei/aurB oocyte injected with wild-type aurB mRNA labeled with α-tubulin (I) show robust furrows which accumulate β-catenin (J), indicating furrow maturation. C,G,K: DAPI images show that nuclear division proceeds normally in all cases.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.