FIGURE

Fig. 1

ID

ZDB-FIG-130211-2

Publication

Nair et al., 2013 - In vitro oocyte culture-based manipulation of zebrafish maternal genes

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Fig. 1

Oogenesis occurs predictably after purging and oocytes of all stages are translation competent. Live images of oocytes. A–P: Oocytes immediately after isolation from purged females on the indicated dpp at time 0 (A,E,I,M) and 2 hr after GFP mRNA injection (B,F,J,N), expressing green fluorescent protein (GFP) protein (C,D,G,H,K,L,O,P). B,F,I,J: Injected Stage I and II (B) and early III (F) oocytes degenerate, while late stage III oocytes (I) mature into stage IV transluscent oocytes (J) with germinal vesicle (arrow). Injected Stage IV oocytes (M) containing germinal vesicle (arrow) mature into stage V oocytes (N, note germinal vesicle breakdown). Scale bar = 300 μm in A–P.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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