ZFIN Image: Nair et al., 2013, Fig. 2

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Figure Caption

Fig. 2 Wild-type aurB mRNA injected into stage IV cei/aurB oocytes rescues the maternal-effect phenotype in cei/aurB embryos. A–L: Animal views of 65 mpf blastodiscs immunolabeled to detect α-tubulin (A,E,I) and β-catenin (B,F,J), with DAPI (4′,6-diamidine-2-phenylidole-dihydrochloride) stainings (C,G,K) and panel merges (D,H,L). A,B,E,F: Wild-type embryos show robust furrows between cells and β-catenin accumulation at the furrows (A,B), while cei/aurB embryo from uninjected cei/aurB stage IV oocyte shows partial, rudimentary furrows (E), which do not accumulate β-catenin (F). I,J: Rescued cei/aurB embryo from cei/aurB oocyte injected with wild-type aurB mRNA labeled with α-tubulin (I) show robust furrows which accumulate β-catenin (J), indicating furrow maturation. C,G,K: DAPI images show that nuclear division proceeds normally in all cases.

Acknowledgments

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