Fig. 9

Different colored GloFish have distinct patterns of fluorescence in their trunk. GloFish with different body colors under room light were assayed for fluorescence emission after excitation by different wavelengths of light. A range of wavelengths was used, and the peak wavelength within each range is listed above each column. Each row contains images of the same fish. The first column has images of the whole fish, and the remaining columns have images of the center dorsal region of the trunk of the fish, with the fish positioned in a lateral view with anterior to the left and dorsal to the top. Brighfield images were taken with a 20 msec exposure, images of fish exposed to 350, 480, 545 nm light were taken at 30 msec exposure, and images of fish exposed to 700 nm light were taken at 200 msec exposure. The exception to this was green fish under 480 nm light, which was taken at a 10 msec exposure because the fluorescence was so bright.