FIGURE

Fig. 7

ID

ZDB-FIG-121212-30

Publication

Lane et al., 2012 - Otx but not mitf transcription factors are required for zebrafish retinal pigment epithelium development

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Fig. 7

The combined knockdown of Otx and Mitf transcription factors creates a more severe phenotype.

(A) The effect of otx1a/otx2 knockdown was compared between wildtype, mitfa^w2 and mitfb^hu3857 single mutants, and mitfb^hu3857;mitfa^w2 double mutants. Morpholinos were injected at a total concentration of 2 ng/embryo using the same needle for all genotypes. Results from one representative experiment are shown. The percentage of severe RPE defects was significantly increased (P<0.0001) in the mitfb^hu3857;mitfa^w2 double mutant background compared to wildtype and single mitfa and mitfb mutants. Wildtype, N = 252; mitfa, N = 196; mitfb, N = 158; mitfa;mitfb, N = 170. (B,C) At 24 hpf dct expression is nearly eliminated in mitfb^hu3857;mitfa^w2 embryos injected with the otx1a/otx2 morpholino combination (C) compared to sibling embryos injected with control morpholino (B).

Expression Data

Genes:	dct otx2a otx2b
Fish:	WT mitfa^w2/w2 mitfb^{hu3857/hu3857} mitfa^w2/w2; mitfb^{hu3857/hu3857} mitfa^w2/w2; mitfb^{hu3857/hu3857} + MO1-otx2a + MO1-otx2b
Knockdown Reagents:	MO1-otx2a MO1-otx2b
Anatomical Terms:	central nervous system optic cup
Stage Range:	20-25 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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