ZFIN ID: ZDB-FIG-121030-32
Hinits et al., 2012 - Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation. Developmental Biology   369(2):199-210 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 10-13 somites to 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 10-13 somites to 20-25 somites

Fig. 2 Early cardiomyocytes fail to differentiate after loss of Mef2c function. A–F. In situ mRNA hybridisation for myl7, vmhc and smyd1b in wild type control (A), mef2cab1086+mef2cb MO (B), mef2cab1086 (C) mef2cb MO (D), mef2cbfh288 (E) and mef2cab1086;mef2cbfh288 (F) embryos, shown in wholemounts in dorsal view, anterior to top. Loss of both mef2ca and mef2cb function greatly reduces myl7, vmhc and smyd1b mRNAs in the bilateral heart fields (arrowheads; A,B and F). Mef2cab1086 mutant embryos have weak myl7 and smyd1b mRNAs early, but recover later, and show no change in vmhc (C). Mef2cb single morphants or mef2cbfh288 mutant show no changes (D). G. Immunostaining for MyHC (A4.1025) in 24 ss mef2cab1086;mef2cbfh288 embryos and their siblings, shown in wholemounts in dorsal view, anterior to top (left panel) and lateral view, anterior to left (right panel). No MyHC is detected in the heart, whereas somitic muscle appears normal (white arrowheads). Scale=100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myh7 WT control 14-19 somites heart primordium ISH
WT + MO2-mef2cb standard conditions 14-19 somites heart primordium ISH
mef2cab1086/+; mef2cbfh288/+ standard conditions 14-19 somites Not Detected heart primordium ISH
mef2cab1086/b1086 standard conditions 14-19 somites heart primordium ISH
mef2cab1086/b1086 + MO2-mef2cb standard conditions 14-19 somites Not Detected heart primordium ISH
mef2cbfh288/fh288 standard conditions 14-19 somites heart primordium ISH
myl7 WT control 14-19 somites heart primordium ISH
WT + MO2-mef2cb standard conditions 14-19 somites heart primordium ISH
mef2cab1086/+; mef2cbfh288/+ standard conditions 14-19 somites Not Detected heart primordium ISH
mef2cab1086/b1086 standard conditions 14-19 somites heart primordium ISH
mef2cab1086/b1086 + MO2-mef2cb standard conditions 14-19 somites Not Detected heart primordium ISH
mef2cbfh288/fh288 standard conditions 14-19 somites heart primordium ISH
smyd1b WT control 10-13 somites heart primordium ISH
14-19 somites heart primordium ISH
WT + MO2-mef2cb standard conditions 10-13 somites heart primordium ISH
14-19 somites heart primordium ISH
mef2cab1086/b1086 standard conditions 10-13 somites Not Detected heart primordium ISH
14-19 somites heart primordium ISH
mef2cab1086/b1086 + MO2-mef2cb standard conditions 10-13 somites Not Detected heart primordium ISH
14-19 somites heart primordium ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-A4.1025 IHC WT standard conditions 20-25 somites heart rudiment
IHC 20-25 somites somite
IHC mef2cab1086/+; mef2cbfh288/+ standard conditions 20-25 somites Not Detected heart rudiment
IHC 20-25 somites somite
Phenotype Details
Fish Conditions Stage Phenotype
mef2cab1086/+; mef2cbfh288/+ standard conditions 14-19 somites cardiac muscle cell differentiation arrested, abnormal
14-19 somites cardiac muscle cell undifferentiated, abnormal
20-25 somites cardiac muscle cell differentiation arrested, abnormal
20-25 somites cardiac muscle cell undifferentiated, abnormal
mef2cab1086/b1086 standard conditions 10-13 somites cardiac muscle cell differentiation arrested, abnormal
10-13 somites cardiac muscle cell undifferentiated, abnormal
14-19 somites cardiac muscle cell differentiation arrested, abnormal
14-19 somites cardiac muscle cell undifferentiated, abnormal
mef2cab1086/b1086 + MO2-mef2cb standard conditions 14-19 somites cardiac muscle cell differentiation arrested, abnormal
14-19 somites cardiac muscle cell undifferentiated, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 369(2), Hinits, Y., Pan, L., Walker, C., Dowd, J., Moens, C.B., and Hughes, S.M., Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation, 199-210, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.