ZFIN Figure: Hinits et al., 2012, Fig. S2

ZFIN ID: ZDB-FIG-121030-23

ZFIN ID: ZDB-FIG-121030-23

Hinits et al., 2012 - Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation. Developmental Biology 369(2):199-210 Full text @ Dev. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Antibody:	Ab2-mef2
Fish:	WT mef2ca^b1086/b1086
Anatomical Terms:	cardiac muscle cell myotome nucleus slow muscle cell nucleus somite slow muscle cell
Stage Range:	20-25 somites to Prim-5

Fig. S2 Mef2c protein is downregulated in mef2ca mutants.
Confocal stacks of Mef2ca/cb immunodetection (Anaspec, red in A-C) and DAPI (blue, A and C) in wildtype and mef2ca mutant embryos. A. Reaction with Mef2c antibody is strong in nuclei of differentiated slow muscle fibres in the somite (left panel), but is missing from the PSM (right panel), and from cranial ganglia indicating that the antibody is not crossreacting with Mef2d and Mef2a protein, respectively. B and C. Anti-Mef2c antibody reacts weakly in CMs of mef2cab1086 mutant embryos (B), but is absent from nuclei in the myotome of the somites (C). Scale = 50 μm (except in A=100 μm).

Gene Expression Details No data available

Antibody Labeling Details

Antibody	Assay	Fish	Conditions	Stage	Qualifier	Anatomy
Ab2-mef2	IHC	WT	standard conditions	20-25 somites		cardiac muscle cell
	IHC			20-25 somites		myotome nucleus
	IHC			Prim-5	Not Detected	cranial ganglion
	IHC			Prim-5	Not Detected	segmental plate
	IHC			Prim-5		slow muscle cell nucleus
	IHC			Prim-5		somite slow muscle cell
	IHC	mef2ca^b1086/b1086	standard conditions	20-25 somites		cardiac muscle cell
	IHC			20-25 somites	Not Detected	myotome nucleus

Phenotype Details No data available

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Developmental Biology, 369(2), Hinits, Y., Pan, L., Walker, C., Dowd, J., Moens, C.B., and Hughes, S.M., Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation, 199-210, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 369(2), Hinits, Y., Pan, L., Walker, C., Dowd, J., Moens, C.B., and Hughes, S.M., Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation, 199-210, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.