FIGURE

Fig. 7

ID

ZDB-FIG-120905-7

Publication

Xu et al., 2012 - Spatiotemporal manipulation of retinoic acid activity in zebrafish hindbrain development via photo-isomerization

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Fig. 7

Local activation of RA via isomerization. Local activation of all-trans RA in zebrafish embryos at 80-90% epiboly (incubated from 4 hpf in 10 μM DEAB) by UV laser illumination at 375 nm following a 5-minute 13-cis RA (cisRA) pulse (5 nM) at 75% epiboly. (A) Schematics describing how embryos were illuminated in localized areas during embryogenesis (see Materials and methods for details). (B) Representative images of embryos incubated in EM (control), in EM plus DEAB, subjected to a 13-cis RA pulse and global UV illumination or to local UV laser-induced isomerization in the tail or head regions. (C) Quantification of the average ratio (Rf) of total fluorescence in r5 versus r3 for the different conditions in B. Error bars indicate s.e.m. Scale bar: 200 μm.

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