FIGURE

Fig. 6

ID

ZDB-FIG-120905-6

Publication

Xu et al., 2012 - Spatiotemporal manipulation of retinoic acid activity in zebrafish hindbrain development via photo-isomerization

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Fig. 6

Partial abortive rescue of hindbrain upon UV illumination of all-trans RA. Hindbrain development in zebrafish embryos expressing GFP in r3 and r5, continuously incubated in 10 μM DEAB from sphere stage (4 hpf) and exposed for 5 minutes to 1 nM all-trans RA (tRA) at sphere followed at various developmental stages by UV illumination for 1 minute (which isomerizes, i.e. deactivates, <80% of all-trans RA). On the left are shown typical patterns of GFP expression at 24 hpf. On the right is shown the average ratio of total fluorescence in r5 versus r3. Note that UV illumination of the embryos until bud stage prevents full rescue of r5. Fuller rescue of hindbrain development by all-trans RA is however possible if UV illumination is performed at the 3-somite stage. Error bars indicate s.e.m. Scale bar: 200 μm.

Expression Data

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