Fig. 3

The trapped gene is a homolog of the tnk1bp1 gene. A. The tnks1bp gene. It encodes 8 exons with the first one (black square), being untranslated. The gene-trap construct landed in the first intron (green arrow), resulting in a product described in (C). Two sets of primers for qRT-PCR were designed to track the presence of alternatively spliced products in transgenic larvae. The amplified products are marked by the two colored lines under the gene depiction. The lines illustrate respectively: red for the product comprised of exon1 (black square) to the gfp from the gene-trap (not depicted), blue for exon1 (black square) to exon2 (blue square of the tnks1bp1 gene. B. Alignment of a stretch of ~70AA of a portion of the C-term of the putative TNKS1BP1 product with ten partial sequences of the putative product of the TNK1BP1 as sequenced/predicted from 10 species. The amino acids highlighted in green are identical and the ones in blue are conservatively substituted. C. Symbolic representation of the mRNA, which was found in abundance in the transgenic embryos. This messenger results from the fusion of the first untranslated exon 1 of the tnks1bp1 gene and the gpf sequence followed by a polyA tail, elements encoded by the integrated transposon construct (green arrow in A). D. Three tail neuromasts are positive (white arrows) by in situ hybridization with a probe against the tnks1bp1 gene. D. and E. Neuromasts after WISH (against tnks1bp1), showing stronger hybridization in the more external cells, corresponding to accessory cells.