FIGURE

Fig. 3

ID

ZDB-FIG-120209-9

Publication

Hall et al., 2012 - Infection-Responsive Expansion of the Hematopoietic Stem and Progenitor Cell Compartment in Zebrafish Is Dependent upon Inducible Nitric Oxide

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Fig. 3

Demand-Driven Granulopoiesis Is Dependent upon Gcsf/Gcsfr Signaling (A) Expression of gcsf within the midbrain and hindbrain of infected larvae (marked by arrows), dorsal views, compared with PBS controls at 1 and 2 dpi. (B) Expression of gcsfr within the AGM of infected larvae, compared with PBS controls at 1 and 2 dpi. (C–E) Dual WMISH of gcsf and lyz, and lcp1 and apoeb, respectively, within the midbrain and hindbrain at 2 dpi following infection, with dorsal views shown. (F) Control MO- and Gcsfr MO-injected Tg(lyz:EGFP)/Tg(gata1:DsRED) larvae following injection of heat-shocked Salmonella (H.S. Sal.), compared with PBS controls at 2 dpi. Numbers represent frequency of embryos or larvae with displayed phenotype. Scale bar, 100 μm in (F). See also Figure S3.

Expression Data

Genes:	apoeb csf3r EGFP lcp1 lyz
Fish:	WT nz117Tg; sd2Tg nz117Tg; sd2Tg + MO4-csf3r
Condition:	bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium
Knockdown Reagent:	MO4-csf3r
Anatomical Terms:	leukocyte macrophage microglial cell neutrophil ventral wall of dorsal aorta cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nz117Tg; sd2Tg
Condition:	bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium
Observed In:	granulocyte differentiation neutrophil
Stage:	Day 4

Phenotype Detail

Acknowledgments

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