Fig. 6
RPE cell movements and origin of RPE subdomains. (A-D2) RPE spreading (13.8-24 hpf). Dorsal view. (A-D) Trajectories over membrane channel average (grayscale). (A2-D2) Trajectories alone. Circles indicate origins. (E,H) Positions of tracked RPE cells at 12 (E) and 24 (H) hpf. Dorsal view. (F,G) Volume rendering of RPE subdomains (12 hpf); posterior (yellow), central (blue), anterior (red). (F) Dorsal view. (G) Lateral view. (I,J) Volume rendering of RPE subdomains (24 hpf). (I) Dorsal view. (J) Lateral view. (K) RPE subdomain trajectories (dorsal view). Circles indicate origins; arrowheads indicate termini. (L,M) Kaede fate mapping. Dorsal views. Volume renderings. (L) OV (12 hpf); photoactivated spot, magenta. (M) Final position after OCM (24 hpf) as indicated by arrowhead. Dashed line outlines OV (12 hpf) and OC (24 hpf). nk, neural keel; br, brain; le, lens. Scale bars: 50 μm. |