FIGURE

Fig. 4

ID

ZDB-FIG-111214-33

Publication

Ma et al., 2011 - Characterization of a Weak Allele of Zebrafish cloche Mutant

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Fig. 4

Definitive hematopoiesis in clo¹⁷² and clo^s5 mutant embryos.

(A–C) stem cell marker c-myb expression at 36 hpf and 3 dpf stage in wild type (A: arrow show VDA region; D: arrow show PBI region), clo¹⁷² (B, E) and clo^s5 mutant (C, F) embryos.(G–I). WISH of βe1 expression at 4 dpf stage in sibling (G: arrow show PBI region), clo¹⁷² (H) and clo mutant (I) embryos. (J–L) WISH to detect l-plastin expression at 4 dpf stage in sibling (J: arrow show PBI region), clo¹⁷² (K) and clo^s5 mutant (L) embryos. (M–O)WISH of T lymophcyte marker rag1 expression at 4 dpf stage in sibling (M: arrow show thymus), clo¹⁷² (N) and clo^s5 mutant (O) embryos. Embryos are shown with anterior to the left and dorsal up. Inserts are high magnification (20×) of the corresponding boxed regions. VDA: ventral wall of dorsa aorta; PBI: posterior blood island.

Expression Data

Genes:	hbbe1.1 lcp1 myb rag1
Fish:	WT npas4l^s5/s5 npas4l^sm0/sm0
Anatomical Terms:	post-vent region erythroid lineage cell post-vent region hematopoietic stem cell post-vent region myeloid cell thymus T cell ventral wall of dorsal aorta erythroid lineage cell ventral wall of dorsal aorta hematopoietic stem cell ventral wall of dorsal aorta myeloid cell
Stage Range:	Prim-25 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	npas4l^s5/s5 npas4l^sm0/sm0
Observed In:	definitive hemopoiesis erythroid lineage cell hematopoietic stem cell myeloid cell T cell
Stage Range:	Prim-25 to Day 4

Phenotype Detail

Acknowledgments

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