FIGURE

Fig. 6

ID

ZDB-FIG-111011-8

Publication

Stevens et al., 2011 - Plasticity of photoreceptor-generating retinal progenitors revealed by prolonged retinoic acid exposure

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Fig. 6

Retinoic acid treatment during retinal neurogenesis results in a significant increase in retinal cell death. Control embryos were treated with DMSO (A, C), or 0.3 μM RA (B, D) at 36 hpf, and either were stained live at 75 hpf with the cell death marker Acridine Orange and prepared as whole mounts for viewing (A, B), or were sectioned at 5 μm and processed for cell death detection with the TUNEL kit (C, D); dorsal is up in all panels. Control embryos showed very few dead/dying cells (arrowheads in A), and these were found predominantly in the inner nuclear layer (C), while those treated with RA (D) showed widespread cell death in the inner nuclear layer (INL), ganglion cell layer (GCL), and circumferential germinal zone (CGZ, D). However, little cell death was detected in the outer nuclear layer (ONL) (D). Bar = 50 μm for all panels.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.