FIGURE

Fig. 4

ID

ZDB-FIG-110804-31

Publication

Lauter et al., 2011 - Two-color fluorescent in situ hybridization in the embryonic zebrafish brain using differential detection systems

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Fig. 4

Control of bleed-through between Fast Red and TSA-FAM detection channels. 24-hpf embryos were hybridized to a dinitrophenol-labeled pax6a probe (A,B) or to a digoxigenin-labeled antisense RNA probe specific for nkx6.1 (C,D). Transcripts were detected using Fast Red (A,B) and TSA-FAM (C,D). Fluorescence signals were recorded in the Fast Red (Ch01: detection of wavelengths greater than 560 nm) and TSA-FAM (Ch02: detection of wavelengths from 505 nm to 545 nm) detection channels. A significant bleed-through of TSA-FAM nkx6.1 signal was detected in the Fast Red detection channel (C). Lateral views with anterior to the left are shown. Images were recorded on a LSM510 confocal microscope and false-colored in ImageJ. Scale bar is 50 μm.

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.