FIGURE

Fig. 2

ID

ZDB-FIG-110804-29

Publication

Lauter et al., 2011 - Two-color fluorescent in situ hybridization in the embryonic zebrafish brain using differential detection systems

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Fig. 2

Effects of hydrogen peroxide and dextran sulfate on Fast Red and Fast Blue detection. 24-hpf embryos were hybridized to a shha dinitrophenyl-labeled RNA probe, which was visualized by AP-Fast Blue (A-D) or AP-Fast Red (E-H) chromogenic staining for 55 min and 80 min, respectively. Lateral views of embryonic brains are shown with anterior to the left. On top of each panel it is indicated whether embryos were permeabilized with 2% hydrogen peroxide (H₂O₂) and whether 5% dextran sulfate (dextran-s) was added to the hybridization buffer. Strongest signals were obtained when hydrogen peroxide treated embryos were hybridized in the presence of dextran sulfate (D,H). Embryos were viewed on an Axioplan II microscope and images were recorded with an Axiocam digital camera. Scale bar is 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.