FIGURE

Fig. 1

ID
ZDB-FIG-110720-57
Publication
White et al., 2011 - A transgenic zebrafish model of targeted oocyte ablation and de novo oogenesis
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Fig. 1

Generation of Tg(zpc:G4VP16/UAS:nfsB-mCherry) transgenic line. A: Ovary-specific G4VP16 mRNA expression was confirmed in adult Tg(zpc:G4VP16) females by RT-PCR. B: Genomic PCR of transgene inserts, zpc:G4VP16 and UAS:nfsB-mCherry, confirming bitransgenic genotype. C: Schematic diagram demonstrating cis-activation of nfsB-mCherry expression by zpc:G4VP16 in Tg(zpc:G4VP16/UAS:nfsB-mCherry) transgenic line. D: nfsB-mCherry expression in Tg(zpc:G4VP16/UAS:nfsB-mCherry) transgenics was confirmed by the detection of mCherry in ovarian tissues (oocytes), and by thepresence of mCherry protein in spawned eggs.
Expression Data
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Anatomical Terms:
Stage Range: 1-cell to Adult

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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