FIGURE SUMMARY
Title

A transgenic zebrafish model of targeted oocyte ablation and de novo oogenesis

Authors
White, Y.A., Woods, D.C., and Wood, A.W.
Source
Full text @ Dev. Dyn.
Fig. 1

Generation of Tg(zpc:G4VP16/UAS:nfsB-mCherry) transgenic line. A: Ovary-specific G4VP16 mRNA expression was confirmed in adult Tg(zpc:G4VP16) females by RT-PCR. B: Genomic PCR of transgene inserts, zpc:G4VP16 and UAS:nfsB-mCherry, confirming bitransgenic genotype. C: Schematic diagram demonstrating cis-activation of nfsB-mCherry expression by zpc:G4VP16 in Tg(zpc:G4VP16/UAS:nfsB-mCherry) transgenic line. D: nfsB-mCherry expression in Tg(zpc:G4VP16/UAS:nfsB-mCherry) transgenics was confirmed by the detection of mCherry in ovarian tissues (oocytes), and by thepresence of mCherry protein in spawned eggs.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Adult
Fig. 2

Ovarian degeneration and regeneration following Mtz treatment. A: Representative micrograph demonstrating gross morphology (external, internal) and ovarian histology of untreated (control) fish; note distended abdomen, large ovarian lobes and abundant oocytes at multiple stages of development throughout ovary. B:Tg(zpc:G4VP16/UAS:nfsB-mCherry) female after 4 days exposure to Mtz (5 mM); external appearance and internal ovarian morphology are similar to that of untreated controls; histologically, analysis reveals evidence of degeneration in large oocytes (*). C:Tg(zpc:G4VP16/UAS:nfsB-mCherry) female after 14 days exposure to Mtz (5 mM); note severe ovarian atrophy, exhibited by loss of abdominal distention and recognizable oocytes. Histological analysis revealed extensive ovarian (oocyte) degeneration, and clusters of early-stage oocytes. D:Tg(zpc:G4VP16/UAS:nfsB-mCherry) female 14 days after Mtz removal (experiment d28); ovarian regeneration is observed. E:Tg(zpc:G4VP16/UAS:nfsB-mCherry) female 21 days after Mtz removal (experiment day 35); external appearance, ovarian morphology and histological features are indistinguishable from controls. Oocyte stages are as described in Selman et al. (1993). Degenerate oocytes are indicated by *; dashed line indicates abdominal morphology; ovarian lobes are outlined. Scale bar = 50 μm.
Fig. 3

Immunohistochemical detection of BrdU incorporation and germline markers in ovarian tissue, indicating germline cell proliferation, following 14 days Mtz treatment (5 mM). A: Clusters of BrdU-positive cells were readily detectable at end of treatment period. B: Immunohistochemical identification of a candidate oogonial stem cell (arrowhead) positive for both BrdU (green) and the germline marker Vasa (Red); arrows indicate BrdU-positive somatic cells.

EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Term:
Stage: Adult
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.
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