PUBLICATION
A transgenic zebrafish model of targeted oocyte ablation and de novo oogenesis
- Authors
- White, Y.A., Woods, D.C., and Wood, A.W.
- ID
- ZDB-PUB-110719-33
- Date
- 2011
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 240(8): 1929-1937 (Journal)
- Registered Authors
- Keywords
- zebrafish, oogenesis, transgenics, ovary, oocyte, stem cell, ablation
- MeSH Terms
-
- Animals
- Animals, Genetically Modified*
- Anti-Infective Agents/pharmacology
- Female
- Male
- Metronidazole/pharmacology
- Models, Animal*
- Oocytes/cytology
- Oocytes/drug effects
- Oocytes/physiology*
- Oogenesis/physiology*
- Ovary/anatomy & histology
- Ovary/drug effects
- Ovary/pathology
- Prodrugs/pharmacology
- Signal Transduction/physiology
- Zebrafish/anatomy & histology
- Zebrafish/physiology*
- PubMed
- 21761478 Full text @ Dev. Dyn.
Citation
White, Y.A., Woods, D.C., and Wood, A.W. (2011) A transgenic zebrafish model of targeted oocyte ablation and de novo oogenesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 240(8):1929-1937.
Abstract
We describe here a novel transgenic zebrafish, Tg(zpc:G4VP16/UAS:nfsB-mCherry) that effectively demonstrates the targeted oocyte ablation in the adult zebrafish ovary. This transgenic line expresses bacterial nitroreductase enzyme (nfsB) under the control of the oocyte-specific zona pellucida C (zpc) gene promoter. Adult transgenic females exposed to the prodrug metronidazole demonstrated near-complete ablation of growing oocytes, resulting in ovarian degeneration and complete cessation of reproductive function. Within 4 weeks of prodrug removal, treated fish demonstrated complete anatomical regeneration of the ovary and, within 7 weeks, ovarian function (fertility) was fully restored. Together, these results demonstrate functional renewal of the oocyte pool in the adult zebrafish ovary. Accordingly, this transgenic zebrafish model system provides a novel means to investigate ovarian growth dynamics in a genetically tractable vertebrate, and may be useful for evaluating signaling interactions that regulate gonadal development processes such as de novo oogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping