Fig. S3

In situ hybridization of c-fos reveals neural activity in basal and induced states. (A) Nominal basal c-fos expression in the brain of adult zebrafish (the hypothalamic region is shown, as boxed in C). (B) Strong and widespread c-fos expression in the brain of zebrafish subjected to intense handling stress (the hypothalamic region is shown, as boxed in C). (C) Section through the diencephalon/midbrain region highlighting the region shown in A and B [corresponding to section 173 in the zebrafish brain atlas (1)]. CIL, central nucleus of the inferior lobe; CM, corpus mamillare; Ctec, commissure tecti; Ctub, commissure of the posterior tuberculum; DIL, diffuse nucleus of the inferior lobe; DTN, dorsal tegmental nucleus; EW, Edinger–Westphal nucleus; FR, fasciculus retroflexus; Hc, caudal zone of periventricular hypothalamus; Hd, dorsal zone of periventricular hypothalamus; LLF, lateral longitudinal fascicle; LR, lateral recess of diencephalic ventricle; MLF, medial longitudinal fascicle; NMLF, nucleus of MLF; PGc, caudal preglomerular nucleus; PGZ, periventricular gray zone of the TeO; Pit, pituitary; PR, posterior recess of diencephalic ventricle; TeO, tectum opticum; TeV, tectal ventricle; TL, torus longitudinalis; TLa, torus lateralis; TPM, tractus pretectomamillaris; TSc, central nucleus of torus semicircularis; TSvl, ventrolateral nucleus of torus semicircularis; TTB, tractus tectobulbaris; Val, lateral division of valvula cerebelli; Vas, vascular lacuna of area postrema.