FIGURE

Fig. 4

ID
ZDB-FIG-110121-50
Publication
Zigman et al., 2011 - Zebrafish Neural Tube Morphogenesis Requires Scribble-Dependent Oriented Cell Divisions
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Fig. 4

Nonautonomous Rescue of Oriented Cross-Midline Cell Division of Single cdh2-/- and mzscrib Mutant Cells

(A–E) Genetic mosaics at neural tube stage (21 hpf) in which donor-derived cells (purple) were transplanted into the presumptive posterior hindbrain of WT host embryos (F-actin in green).

(A) Bilateral distribution of WT cells (arrows) in a WT environment.

(B) cdh2-/- cells form unilateral aggregates in a WT environment.

(C) In contrast, isolated cdh2-/- progenitors show rescued bilateral distribution and normal cell shape.

(D) A large group of mzscrib mutant cells in a WT environment with unilateral cell distribution.

(E) Isolated mzscrib cells in a WT environment have rescued shape and bilateral distribution.

(F) Utr-CH-RFP reporter in mosaic embryos showing normal apical enrichment in WT control transplants, loss of apical enrichment in a cluster of transplanted cdh2-/- cells, and rescue in a single cdh2-/- cell that is surrounded by WT host cells. Images represent maximum-intensity projections of optical sections.

(G) Quantification of occurrence of abnormal F-actin organization in WT (black; n = 5 experiments, 13 embryos), mzscrib (blue; n = 3 experiments, 21 embryos), and cdh2-/- (red; n = 2 experiments, 15 embryos) mosaics.

Embryos are shown in dorsal view with anterior to the top. Dotted white line indicates the neural tube midline; two-way arrows indicate the apicobasal axis of the neuroepithelium. See also Figure S4.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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