FIGURE

Fig. 4

ID

ZDB-FIG-101207-23

Publication

Teh et al., 2010 - Optogenetic in vivo cell manipulation in KillerRed-expressing zebrafish transgenics

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Fig. 4

Illumination of KR-expressing heart bleaches KR and causes cardiac edema.(A-B) KR expression in the 3dpf beating heart of SqKR15 was reduced to 21% after 5 min of exposure to intense green light. (A) Expression of GFP and KR in the ET33-mi3A:SqKR15 double transgenic embryo prior to illumination and (B) after illumination. (C-D) Larvae at 4 dpf, one day after illumination: C - ET33-mi3A (control), D - ET33-mi3A:SqKR15 (experimental sample). Cardiac edema developed in ET33-mi3A:SqKR15 larvae one day after illumination (D). Apoptosis in the heart of SqKR15 is similar to that in control.

Expression Data

Genes:	EGFP KillerRed
Fish:	sqet33mi3AEt; sqKR15Et
Condition:	high light intensity, lighting conditions
Anatomical Term:	heart
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sqet33mi3AEt; sqKR15Et
Condition:	high light intensity, lighting conditions
Observed In:	atrial myocardium heart pericardium
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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