FIGURE

Fig. 1

ID
ZDB-FIG-101011-59
Publication
Oteiza et al., 2010 - Planar cell polarity signalling regulates cell adhesion properties in progenitors of the zebrafish laterality organ
Other Figures
All Figure Page
Back to All Figure Page
Fig. 1

The PCP signalling components wnt11 and prickle1a are expressed in the DFCs and control KV morphogenesis. (A-C′) Expression of wnt11, pk1a and wnt5 in wild-type zebrafish embryos at 75% epiboly as revealed by wholemount in situ hybridisation. Arrows in dorsal views (A,B,C) and arrowheads in sagittal sections (A2,B2,C2) point to the dorsal forerunner cell (DFC) expression domain. (D-I) Kuppfer′s vesicle (KV) lumen morphologies at the 8- to 10-somite stage in wild-type (D,G) and wnt11- and pk1a-defective (E,F,H,I) embryos. (D-F) DIC microscopy views of KV in living embryos. (G-I) Three-dimensional projections of confocal stacks through the entire KV stained with anti-ZO-1 antibody, outlining lumen shape. (J) Horizontal bar chart of the relative frequency of the three main KV morphological phenotypes (large single lumen, yellow; small mis-shapen lumen, blue; fragmented lumen, red) scored in different experimental conditions: control (wild-type untreated, n=21); slb [wnt11 (silberblick) mutants, n=12]; pk1a-MO 3 ng (wild-type injected with 3 ng of pk1a-MO at the 1-cell stage, n=9); MZppt [maternal zygotic wnt5 (pipetail) mutants, n=10]; slb;ppt (wnt11;slb and wnt5;ppt double mutants, n=14); pk1a-MO 1.5 ng (wild-type injected with 1.5 ng of pk1a-MO at the 1-cell stage, n=8); slb;pk1a-MO 1.5 ng (slb mutants injected with 1.5 ng of pk1a-MO at the 1-cell stage, n=19); MZppt;pk1a-MO 1.5 ng (MZppt injected with 1.5 ng of pk1a-MO at the 1-cell stage, n=10); pk1a-MO 3 ng DFC [abrogation of pk1a in wild-type DFCs by injection of 3 ng of pk1a-MO in the yolk syncitial layer (YSL) at mid-blastula stages, n=18]; and slb;pk1a-MO 3 ng DFC (abrogation of pk1a in slb DFCs by injection of 3 ng of pk1a-MO in the YSL at midblastula stages, n=10). Scale bars: 20 μm.

Expression Data
Genes:
Fish:
Anatomical Term:
Stage: 75%-epiboly

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: 5-9 somites

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development