Fig. S5
- ID
- ZDB-FIG-100309-23
- Publication
- Nojima et al., 2010 - Syntabulin, a motor protein linker, controls dorsal determination
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Detection of the insertion by Southern blotting. (A) Schematic representation of the zebrafish syntabulin gene structure and location of the probe used for Southern blotting analysis. (B) Southern blot. Genomic DNA (10 μg) was isolated from four wild-type and four tkkrk4/rk4 adult fish and digested with EcoRI, followed by Southern hybridization with the probe described in A. A larger than 10 kb band was detected in the wild-type genome, whereas a <7 kb band was detected in the tkk genome (asterisks), indicating alteration of the 5′ syntabulin genomic sequence. |