FIGURE

Fig. 2

ID

ZDB-FIG-100225-19

Publication

Rai et al., 2010 - Dnmt3 and G9a cooperate for tissue-specific development in zebrafish

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Fig. 2

Distinct tissue-specific developmental defects in dnmt1 and dnmt3 morphants. A, C, and E, whole mount in situ analysis of dhand (at 48 hpf) (A), fabp2, trypsin, fabp10, insulin, and trypsin (C), and irbp (E) (all at 96 hpf) in dnmt1, dnmt3, and control morphants. In panel A, arrows show loss of last two pharyngeal pouches in dnmt3 morphants. In panel C, arrows point to expression of gata6 in the pancreas. B, Alcian Blue staining of Control, dnmt1, or dnmt3 morphants at 96 hpf shows loss of jaw structure in dnmt1 morphants but not in dnmt3 morphants. D, toluidine blue staining of histological cross-sections within the dnmt3 morphant retinas at 80 hpf. Note the loss of organization of different retinal layers in dnmt3 morphants. F, quantitative RT-PCR for Opsin Shortwave 1 and Opsin Shortwave 2 in control, dnmt1, or dnmt3 morpholino-injected embryos at 96 hpf.

Expression Data

Genes:	fabp2 fabp10a gata6 hand2 ins opn1sw1 opn1sw2 prss1 rbp3
Fish:	WT WT + MO1-dnmt3bb.2 WT + MO4-dnmt1
Knockdown Reagents:	MO1-dnmt3bb.2 MO4-dnmt1
Anatomical Terms:	endocrine pancreas exocrine pancreas intestine liver pancreas pharyngeal pouch pharyngeal pouches 2-6 retina retinal pigmented epithelium
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-dnmt3bb.2 WT + MO4-dnmt1
Knockdown Reagents:	MO1-dnmt3bb.2 MO4-dnmt1
Observed In:	camera-type eye photoreceptor cell differentiation digestive tract development embryonic viscerocranium morphogenesis eye lens retina retina layer formation retinal pigmented epithelium dorsal region retinal pigmented epithelium ventral region
Stage Range:	Long-pec to Day 4

Phenotype Detail

Acknowledgments

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Open Access.

Full text @ J. Biol. Chem.