ZFIN ID: ZDB-FIG-091113-21
Furutani-Seiki et al., 1996 - Neural degeneration mutants in the zebrafish, Danio rerio. Development (Cambridge, England)   123:229-239 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to 14-19 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-25 to Day 4

Fig. 2

Class I mutant phenotypes. (A,C,E,G,I,K,M) Wild type; (B,D,F,H,J) aoi, (L) tc294a and (N) ty103a. (A-H) at 36 h; (I-L) at the 10- somite stage and (M,N) at the 15-somite stage. (C,D) Acridine orange (AO)-stained embryos of (A,B) respectively. (G,H) Parasagittal section of the tectum stained with toluidine blue. In situ hybridization with Zash 1a probe (I-L) and with Zash 1b probe (M,N). Apoptotic cells are indicated by white arrowheads. In aoi mutants apoptotic cells are present in the tectum (D,H arrowhead), cerebellar fold, dorsal hindbrain (F) and the neural retina (D arrowhead). Normal olfactory epithelium is stained with acridine orange (C, arrow). (I,J) The dorsal forebrain expression (arrow) and rhombomere 2 expression of Zash 1a (arrowhead) are reduced in aoi mutants whereas ventral midbrain expression (black star) is increased; (K,L) the ventral forebrain expression (white star), tegmentum expression (black star) are increased whereas the midbrain-hindbrain boundary expression of Zash 1a (arrowhead) is reduced in tc294a embryos; (M,N) the telencephalic expression of Zash 1b (arrow) is increased in contrast to the absence of its midbrain expression (arrowhead) in ty103a. Scale bar, approximately 100 µm in A-D and IN; 200 µm in E and F; 300 µm in G and H.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ascl1a WT standard conditions 10-13 somites basal plate midbrain region ISH
10-13 somites forebrain dorsal region ISH
10-13 somites forebrain ventral region ISH
10-13 somites midbrain ventral region ISH
10-13 somites midbrain hindbrain boundary neural rod ISH
10-13 somites rhombomere 2 ISH
aoitr222d/tr222d standard conditions 10-13 somites basal plate midbrain region ISH
10-13 somites forebrain dorsal region ISH
10-13 somites forebrain ventral region ISH
10-13 somites midbrain ventral region ISH
10-13 somites midbrain hindbrain boundary neural rod ISH
10-13 somites rhombomere 2 ISH
unm_tc294atc294a/tc294a standard conditions 10-13 somites basal plate midbrain region ISH
10-13 somites forebrain dorsal region ISH
10-13 somites forebrain ventral region ISH
10-13 somites midbrain ventral region ISH
10-13 somites midbrain hindbrain boundary neural rod ISH
10-13 somites rhombomere 2 ISH
ascl1b WT standard conditions 14-19 somites midbrain ISH
14-19 somites telencephalon ISH
unm_ty103aty103a/ty103a standard conditions 14-19 somites Not Detected midbrain ISH
14-19 somites telencephalon ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
aoitr222d/tr222d standard conditions Prim-25 apoptotic process increased occurrence, abnormal
Prim-25 brain degenerate, abnormal
Prim-25 cerebellum apoptotic, abnormal
Prim-25 hindbrain dorsal region apoptotic, abnormal
Prim-25 neuroepithelial cell apoptotic, abnormal
Prim-25 optic tectum apoptotic, abnormal
Prim-25 retinal neural layer apoptotic, abnormal
recte356a/te356a standard conditions Day 4 cerebellum degenerate, abnormal
Day 4 eye decreased size, abnormal
Day 4 hindbrain dorsal region degenerate, abnormal
Day 4 optic tectum degenerate, abnormal
Acknowledgments:
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