ZFIN ID: ZDB-FIG-090501-39
Roussign√© et al., 2009 - Nodal signalling imposes left-right asymmetry upon neurogenesis in the habenular nuclei. Development (Cambridge, England)   136(9):1549-1557 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage Range: Prim-15 to Prim-25
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-15 to Prim-25

Fig. 6 Nodal signalling promotes early asymmetric neurogenesis. (A-D) Confocal projections of Tg(huC:gfp) transgenic zebrafish embryos incubated with DMSO (A,C) or with SB431542 (B,D) from 10 hpf and fixed at 34 (A,B) or 38 (C,D) hpf. Arrows indicate that the left pools of HuC+ neurons are bigger than those on the right in DMSO-treated embryos (A,C), whereas the left and right pools are symmetric in embryos incubated with SB431542 (B,D). The epiphysis is indicated by a blue line. Habenular neurogenesis is also delayed in SB431542-treated embryos. (E-H) Numbers of left and right HuC+ habenular neurons (E,G) and corresponding AI (F,H) in embryos treated with DMSO or SB431542 at 10 (E,F) or 16 (G,H) hpf. Habenular neurons were detected in Tg(huC:gfp) transgenic embryos using an anti-GFP (E) or HuC/D (G) antibody. A symmetry line (black) is included as a reference in E and G. Dotted lines represent best-fit linear regressions. Horizontal black lines in F and H indicate the median AI value for the group of like-treated embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP knu3Tg control Prim-15 epithalamus IHC
Prim-25 epithalamus IHC
knu3Tg chemical treatment: pharmaceutical Prim-15 epithalamus IHC
Prim-25 epithalamus IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
knu3Tg chemical treatment: pharmaceutical Prim-15 habenula determination of left/right symmetry disrupted, abnormal
Prim-15 habenula neurogenesis delayed, abnormal
Prim-15 habenula neurogenesis process quality, abnormal
Prim-15 nodal signaling pathway involved in determination of left/right asymmetry disrupted, abnormal
Prim-25 habenula determination of left/right symmetry disrupted, abnormal
Prim-25 habenula neurogenesis delayed, abnormal
Prim-25 habenula neurogenesis process quality, abnormal
Prim-25 nodal signaling pathway involved in determination of left/right asymmetry disrupted, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development