ZFIN ID: ZDB-FIG-090501-36
Roussign√© et al., 2009 - Nodal signalling imposes left-right asymmetry upon neurogenesis in the habenular nuclei. Development (Cambridge, England)   136(9):1549-1557 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Long-pec to Pec-fin

Fig. 2 cxcr4b is a marker of habenular progenitors/early-born neurons. (A-B″) Single confocal images (dorsal views) of a 48 hpf Tg(huC:gfp) zebrafish embryo (A-A″) with in situ labelling of ngn1 and a 48 hpf Tg(ngn1:gfp) embryo (B-B″) with in situ labelling of cxcr4b. (A″-B″) Merged images. The habenulae are indicated by a dashed white line. (C-E) Cross-sections of double-labelled habenulae generated from confocal z-series. (C) Immuno-detection of GFP protein (green) and localisation of cxcr4b transcripts (red) in a 48 hpf Tg(huC:gfp) embryo. (D) Immuno-detection of GFP protein (green) and localisation of ngn1 mRNA (red) in a 48 hpf Tg(huC:gfp) transgenic embryo. (E) Immuno-detection of BrdU (red) and GFP protein (green) in a 60 hpf Tg(huC:gfp) embryo pulsed with BrdU at 48 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cxcr4b knu3Tg standard conditions Long-pec habenula ISH
sb1Tg standard conditions Long-pec habenula ISH
EGFP knu3Tg standard conditions Long-pec habenula IHC
Pec-fin habenula IHC
GFP sb1Tg standard conditions Long-pec habenula IHC
neurog1 knu3Tg standard conditions Long-pec habenula ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development