ZFIN ID: ZDB-FIG-090408-63 |
Genes: | |
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Fish: | |
Anatomical Terms: | |
Stage Range: | 10-13 somites to 14-19 somites |
Fig. 3 sim1 and olig2 are co-expressed in the prospective posterior tuberculum. A-L: Spatial expression analyses of sim1 in transgenic reporter lines expressing GFP, which is driven by the respective olig2 (olig2::GFP) or neurog1 (neurog1::GFP) promoters. The expression of sim1 mRNA was visualized using fluorescent in situ hybridiztion (red) followed by detection of either olig2- or neurog1-expressing cells with anti-GFP antibody. For the purpose of clarity, the expression of neurog1::GFP is visualized in pseudo color (blue). M-R: Whole mount fluorescent in situ hybridiztion of neurog1 (blue, pseudo color) followed by immunofluorescent staining of GFP, which marks olig2-expressing cells (green). A-C, G-I, M-O: Lateral view, anterior to the left; D-F, J-L, P-R: dorsal view, anterior to the left. dTha, dorsal thalamus; PT, posterior tuberculum; Teg, tegmentum; Tel, telencephalon Tha, thalamus; vTha, ventral thalamus. Scale bar = 36 μm. |
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
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EGFP | vu12Tg | standard conditions | 10-13 somites | caudal tuberculum | IHC | |
10-13 somites | dorsal thalamus | IHC | ||||
14-19 somites | caudal tuberculum | IHC | ||||
14-19 somites | dorsal thalamus | IHC | ||||
GFP | sb1Tg | standard conditions | 14-19 somites | caudal tuberculum | IHC | |
14-19 somites | ventral thalamus | IHC | ||||
neurog1 | vu12Tg | standard conditions | 10-13 somites | caudal tuberculum | ISH | |
10-13 somites | ventral thalamus | ISH | ||||
sim1a | sb1Tg | standard conditions | 14-19 somites | caudal tuberculum | ISH | |
14-19 somites | ventral thalamus | ISH | ||||
vu12Tg | standard conditions | 10-13 somites | caudal tuberculum | ISH | ||
10-13 somites | ventral thalamus | ISH | ||||
14-19 somites | caudal tuberculum | ISH | ||||
14-19 somites | ventral thalamus | ISH |
Acknowledgments: | This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn. |
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