ZFIN ID: ZDB-FIG-090408-63
Borodovsky et al., 2009 - Neural protein Olig2 acts upstream of the transcriptional regulator sim1 to specify diencephalic dopaminergic neurons. Developmental dynamics : an official publication of the American Association of Anatomists   238(4):826-834 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to 14-19 somites

Fig. 3 sim1 and olig2 are co-expressed in the prospective posterior tuberculum. A-L: Spatial expression analyses of sim1 in transgenic reporter lines expressing GFP, which is driven by the respective olig2 (olig2::GFP) or neurog1 (neurog1::GFP) promoters. The expression of sim1 mRNA was visualized using fluorescent in situ hybridiztion (red) followed by detection of either olig2- or neurog1-expressing cells with anti-GFP antibody. For the purpose of clarity, the expression of neurog1::GFP is visualized in pseudo color (blue). M-R: Whole mount fluorescent in situ hybridiztion of neurog1 (blue, pseudo color) followed by immunofluorescent staining of GFP, which marks olig2-expressing cells (green). A-C, G-I, M-O: Lateral view, anterior to the left; D-F, J-L, P-R: dorsal view, anterior to the left. dTha, dorsal thalamus; PT, posterior tuberculum; Teg, tegmentum; Tel, telencephalon Tha, thalamus; vTha, ventral thalamus. Scale bar = 36 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP vu12Tg standard conditions 10-13 somites caudal tuberculum IHC
10-13 somites dorsal thalamus IHC
14-19 somites caudal tuberculum IHC
14-19 somites dorsal thalamus IHC
GFP sb1Tg standard conditions 14-19 somites caudal tuberculum IHC
14-19 somites ventral thalamus IHC
neurog1 vu12Tg standard conditions 10-13 somites caudal tuberculum ISH
10-13 somites ventral thalamus ISH
sim1a sb1Tg standard conditions 14-19 somites caudal tuberculum ISH
14-19 somites ventral thalamus ISH
vu12Tg standard conditions 10-13 somites caudal tuberculum ISH
10-13 somites ventral thalamus ISH
14-19 somites caudal tuberculum ISH
14-19 somites ventral thalamus ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.