FIGURE

Fig. 5

ID

ZDB-FIG-080516-20

Publication

Shin et al., 2008 - Multiple roles for Med12 in vertebrate endoderm development

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Fig. 5

Med12 regulates her5 expression in endodermal cells during gastrulation and somitogenesis. (A, A′) In situ hybridization at 90% epiboly shows specific reduction of her5 expression in endodermal cells in med12^s432 mutants compared to wild-type siblings. The V shaped stripe represents the domain of her5 expression in the midbrain–hindbrain boundary. (B–D′) Injection of med12 MO into the Tg(-0.7her5:EGFP)^ne2067 line caused decreased GFP expression. Note that the Tg(-0.7her5:EGFP)^ne2067 line shows GFP expression in the pharyngeal endoderm as well as the organ-forming region during late somitogenesis stages (C, D). White bracket in C–D′ indicates pharyngeal region. Exposure time for imaging B, B′ was 300 ms; C, C′ was 250 ms; D, D′ was 250 ms. Dorsal views, anterior to the top.

Expression Data

Genes:	EGFP her5
Fish:	WT med12^s432/s432 ne2067Tg ne2067Tg + MO5-med12
Knockdown Reagent:	MO5-med12
Anatomical Terms:	digestive system endoderm endodermal cell midbrain hindbrain boundary neural plate pharyngeal endoderm
Stage Range:	90%-epiboly to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 317(2), Shin, C.H., Chung, W.S., Hong, S.K., Ober, E.A., Verkade, H., Field, H.A., Huisken, J., and Stainier, D.Y., Multiple roles for Med12 in vertebrate endoderm development, 467-479, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.