FIGURE

Fig. 6

ID

ZDB-FIG-071116-5

Publication

Wingert et al., 2007 - The cdx Genes and Retinoic Acid Control the Positioning and Segmentation of the Zebrafish Pronephros

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Fig. 6

cdx Genes Regulate the Expression Boundaries of raldh2 and cyp26a1. (A) Expression of raldh2 and cyp26a1 in wild-type, cdx4^–/– mutants, and cdx-deficient embryos by whole-mount in situ hybridization. Brackets indicate raldh2 and cyp26a1 expression domains in the anterior paraxial mesoderm of 5 somite stage embryos. Right column is an overlay of the raldh2 and cyp26a1 expression patterns, arrows indicate the anterior boundary of the presumptive RA source. (B) Double whole-mount in situ hybridization showing co-staining of krox20, myoD, raldh2, and cyp26a1 at 10 somites in wild-type, cdx4^–/– mutants, and cdx-deficient embryos (gene names are color-coded to match their staining product). Brackets and numbers indicate raldh2 and cyp26a1 expression domains and somite position, respectively.

Expression Data

Genes:	aldh1a2 cyp26a1 egr2b myod1
Fish:	TU cdx4^{tv205c/tv205c} (TU) cdx4^{tv205c/tv205c} + MO2-cdx1a (TU)
Knockdown Reagent:	MO2-cdx1a
Anatomical Terms:	paraxial mesoderm rhombomere 3 rhombomere 5 somite tail bud
Stage Range:	5-9 somites to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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