ZFIN ID: ZDB-FIG-071116-12
Blanco et al., 2007 - Snail1a and Snail1b cooperate in the anterior migration of the axial mesendoderm in the zebrafish embryo. Development (Cambridge, England)   134(22):4073-4081 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Shield to Bud
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 50%-epiboly to Prim-15

Fig. 1 snail1b knockdown induced morphological defects at gastrulation and segmentation stages. (A) Two different morpholino antisense oligonucleotides were designed against adjacent sequences in the 5' region of the snail1b cDNA. (B,D) Embryos at mid-gastrulation and (C,E) at the 18-somite stage. Morpholino injection (3 ng/embryo) produced defects in extension movements at early embryonic stages that led to the shortening of the body axis at 30 hpf (compare H with I). (F,G,J) This phenotype was rescued by co-injection of snail1b mRNA. (K,L,N,O,P,R) snail1a but not snail1b was prominently expressed in the involuting and posterior mesoderm. (M,Q) Lateral views highlight the expression of snail1a in the posterior half of the embryo and snail1b in the anterior. The dotted lines indicate the position of the margin and the adaxial cells (P) and that of the prechordal plate (R). ac, adaxial cells; ame, anterior axial mesendoderm; cm, condensing mesenchyme; pm, paraxial mesoderm; tbm, tail bud mesenchyme.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
snai1a WT standard conditions Shield margin ISH
75%-epiboly margin ISH
Bud adaxial cell ISH
Bud margin ISH
Bud paraxial mesoderm ISH
Bud tail bud ISH
snai1b WT standard conditions Shield Not Detected margin ISH
75%-epiboly Not Detected margin ISH
75%-epiboly presumptive cephalic mesoderm ISH
Bud adaxial cell ISH
Bud axial mesoderm ISH
Bud Not Detected prechordal plate ISH
Bud presumptive cephalic mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-snai1b standard conditions 50%-epiboly convergent extension involved in axis elongation disrupted, abnormal
14-19 somites post-vent region decreased length, abnormal
14-19 somites trunk decreased length, abnormal
14-19 somites whole organism anterior-posterior axis decreased length, abnormal
Prim-15 post-vent region decreased length, abnormal
Prim-15 trunk decreased length, abnormal
Prim-15 whole organism anterior-posterior axis decreased length, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development