FIGURE

Fig. 5

ID

ZDB-FIG-070810-15

Publication

Chong et al., 2007 - The chemokine, Sdf-1, and its receptor, Cxcr4, are required for formation of muscle in zebrafish

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Fig. 5

Knockdown of E-box factors affects cxcr4a and sdf1a transcription. Dorsal views (A-F). Embryos between 13–14 h were analyzed. (A,D) Control showing cxcr4a (n = 20) and sdf1a (n = 20) expression respectively. (B,E) Double myoD-myf5 morphants demonstrate reduction of cxcr4a (n = 15/20) and sdf1a (n = 16/20) transcription respectively. This indicates cooperative function of MyoD and Myf5. (C,F) e12 morphants have vast reduction of cxcr4a (n = 20/20) and sdf1a (n = 20/20) transcription, this confirms that E12 is a major regulating factor. Black arrow in F indicates sdf1a staining in the non somitic lateral mesoderm. Abbreviation: n – notochord.

Expression Data

Genes:	cxcl12a cxcr4a
Fish:	WT WT + MO1-tcf3a WT + MO4-myf5 + MO4-myod1
Knockdown Reagents:	MO1-tcf3a MO4-myf5 MO4-myod1
Anatomical Terms:	lateral mesoderm somite
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-tcf3a WT + MO4-myf5 + MO4-myod1
Knockdown Reagents:	MO1-tcf3a MO4-myf5 MO4-myod1
Observed In:	somite
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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