ZFIN ID: ZDB-FIG-050719-9
Zecchin et al., 2005 - Expression analysis of jagged genes in zebrafish embryos. Developmental dynamics : an official publication of the American Association of Anatomists   233(2):638-645 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5

Fig. 3 jagged1b expression pattern. In situ hybridization of embryos at different developmental stages performed with the jagged1b probe. A: At the 10-somite stage (10s), jagged1b is expressed in the notochord and the pronephros primordia. B-D: At the 20-somite stage, jagged1b-expressing cells are detected mainly in the lens, otic vesicles, and two bilateral groups of cells situated anteriorly and posteriorly to the latter (B), in the caudal part of the notochord (C), and in the rostral part of the pronephric ducts (D). C: At the 20-somite stage, jagged1b transcripts are detected in a group of cells located ventrally and posteriorly to the anus. E: At 24 hours postfertilization (hpf), jagged1b and pax2a are coexpressed in cells of the rostral end of the pronephric duct and lateral part of the nephron primordia, but only jagged1b is expressed in the medial cells of the nephron primordia. F: At the same stage, jagged1b transcripts are also expressed in the region extending from the otic vesicle to the eye. G,H: At 28 hpf, the jagged1b (arrow) expression domain does not overlap with that of glucagon in the pancreatic islet (G), but is in part overlapped with the pdx1 expression domain (H). Views are dorsal (A,B,F), lateral (C,H), or ventral (D,E,G). Anterior is to the left in all pictures. a, anus; ep, endocrine pancreas; l, lens; n, notochord; np, nephron primordia; ov, otic vesicle; pd, pronephric duct; pdp, pronephric duct primordium; r3, rhombomere 3; r5, rhombomere 5.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
egr2b WT standard conditions Prim-5 rhombomere 3 ISH
Prim-5 rhombomere 5 ISH
jag1b WT standard conditions 10-13 somites notochord ISH
10-13 somites pronephric duct ISH
20-25 somites Not Detected notochord ISH
20-25 somites otic vesicle ISH
20-25 somites pronephric duct ISH
20-25 somites solid lens vesicle ISH
Prim-5 otic vesicle ISH
Prim-5 posterior pancreatic bud ISH
Prim-5 pronephric duct ISH
pax2a WT standard conditions Prim-5 pronephric duct ISH
pdx1 WT standard conditions Prim-5 posterior pancreatic bud ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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