CRISPR

CRISPR1-chst6

ID

ZDB-CRISPR-250522-7

Name

CRISPR1-chst6

Previous Names

None

Target

chst6 (1)

Sequence

5' - GCTGGAGAGTGTCGAAGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ibg1	chst6
ibg2	chst6
ibg3	chst6

Expression

Gene expression in Wild Types + CRISPR1-chst6

No data available

Phenotype

Phenotype resulting from CRISPR1-chst6

No data available

Phenotype of all Fish created by or utilizing CRISPR1-chst6

Phenotype	Fish	Conditions	Figures
head deformed, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
whole organism ab1-5d4 labeling decreased amount, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 4 from Basol et al., 2024
corneal stroma Ab1-tgfbi labeling absent, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 8 from Basol et al., 2024
head deformed, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
ceratohyal cartilage increased angle to ceratohyal cartilage, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 5 from Basol et al., 2024
corneal stroma condensed, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
pericardium edematous, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
pericardium edematous, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
cornea ab1-5d4 labeling absent, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 4 from Basol et al., 2024
caudal fin absence of anatomical entity, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 5 from Basol et al., 2024
cornea opaque, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 6 from Basol et al., 2024
vertebral column kinked, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 5 from Basol et al., 2024
corneal stroma absence of anatomical entity, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
otic vesicle ab1-5d4 labeling decreased distribution, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 4 from Basol et al., 2024
corneal stroma posterior region degenerate, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
caudal fin decreased width, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 5 from Basol et al., 2024
brain ab1-5d4 labeling decreased distribution, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 4 from Basol et al., 2024
trunk curved, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
trunk curved, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
visual behavior decreased process quality, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 6 from Basol et al., 2024
yolk edematous, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
Descemet's membrane degenerate, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
yolk edematous, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
post-vent region bent, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
corneal stroma decreased thickness, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
corneal stroma ab1-5d4 labeling absent, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 8 from Basol et al., 2024
post-vent region bent, abnormal	chst6^ibg1/ibg1 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
mouth open, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 5 from Basol et al., 2024
corneal stroma Ab1-tgfbi labeling absent, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 8 from Basol et al., 2024
whole organism ab1-5d4 labeling decreased amount, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 4 from Basol et al., 2024
vertebral column kinked, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 5 from Basol et al., 2024
corneal stroma condensed, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
head deformed, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
head deformed, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
cornea ab1-5d4 labeling absent, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 4 from Basol et al., 2024
caudal fin absence of anatomical entity, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 5 from Basol et al., 2024
cornea opaque, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 6 from Basol et al., 2024
pericardium edematous, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
pericardium edematous, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
corneal stroma posterior region degenerate, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
corneal stroma absence of anatomical entity, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
caudal fin decreased width, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 5 from Basol et al., 2024
trunk curved, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
trunk curved, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
corneal stroma decreased thickness, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
yolk edematous, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
yolk edematous, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
Descemet's membrane degenerate, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
post-vent region bent, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
corneal stroma ab1-5d4 labeling absent, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 8 from Basol et al., 2024
post-vent region bent, abnormal	chst6^ibg2/ibg2 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
post-vent region bent, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
mouth open, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 5 from Basol et al., 2024
whole organism ab1-5d4 labeling decreased amount, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 4 from Basol et al., 2024
vertebral column kinked, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 5 from Basol et al., 2024
corneal epithelium irregularly shaped, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
corneal epithelium increased thickness, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
corneal stroma condensed, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
ceratohyal cartilage decreased angle to ceratohyal cartilage, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 5 from Basol et al., 2024
head deformed, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
head deformed, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
cornea opaque, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 6 from Basol et al., 2024
mouth protruding, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 5 from Basol et al., 2024
cornea ab1-5d4 labeling absent, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 4 from Basol et al., 2024
caudal fin absence of anatomical entity, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 5 from Basol et al., 2024
pericardium edematous, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
pericardium edematous, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
corneal stroma posterior region degenerate, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
otic vesicle ab1-5d4 labeling decreased distribution, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 4 from Basol et al., 2024
corneal stroma absence of anatomical entity, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
brain ab1-5d4 labeling decreased distribution, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 4 from Basol et al., 2024
visual behavior decreased process quality, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 6 from Basol et al., 2024
caudal fin decreased width, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 5 from Basol et al., 2024
trunk curved, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
trunk curved, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
corneal stroma decreased thickness, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
yolk edematous, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
Descemet's membrane degenerate, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 7 from Basol et al., 2024
yolk edematous, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
corneal stroma ab1-5d4 labeling absent, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 8 from Basol et al., 2024
corneal stroma Ab1-tgfbi labeling decreased distribution, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 8 from Basol et al., 2024
post-vent region bent, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 3 from Basol et al., 2024
ceratobranchial cartilage increased distance ceratobranchial cartilage, abnormal	chst6^ibg3/ibg3 (AB)	standard conditions	Fig. 5 from Basol et al., 2024

Citations