Acute LSD effect in the open field (OF) test. (A) Time spent in inner zone was not significantly different after acute dosing. (B) Time in transition zone was not significantly different after acute dosing. (C). Time spent in thigmotaxis zone was not significantly different after acute dosing. (D) Velocity was significantly different after acute dosing. The * represents a significant difference of P < 0.05. (E) Meandering was not significantly different after acute dosing. (F) High mobility was significantly different after acute dosing. The * represents a significant difference of P < 0.05, ** represents a significant difference of P < 0.01, and *** represents a significant difference of P < 0.001. (G) Mobility was not significantly different after acute dosing. (H) Immobility was not significantly different after acute dosing. Sample sizes for A-H: 0 (n = 10), 1.5 (n = 10), 15 (n = 10), 150 (n = 10). Data presented as mean + /- SD.

Acute LSD effect in the novel object approach (NOA) test. (A) Time spent in inner zone was not significantly different during acute dose NOA testing. (B) Time in transition zone was significantly different between groups during acute dose NOA testing. The ** represents a significant difference of P < 0.01. (C). Time spent in thigmotaxis was significantly different between groups during acute dose NOA testing. The ** represents a significant difference of P < 0.01. (D) Velocity was not significantly different during acute dose NOA testing. (E) Meandering was significantly different during acute dose NOA testing, but there were no post-hoc differences. (F) High mobility was not significantly different during NOA acute dose testing. (G) Mobility was not significantly different during the acute dose NOA testing. (H) Immobility was not significantly different during NOA acute dose testing with a difference between 0 µg/L and 15 µg/L. The * represent a significant difference of P < 0.05. Sample sizes for A-H: 0 (n = 10), 1.5 (n = 10), 15 (n = 10), 150 (n = 10). Data presented as mean + /- SD.

Repeated LSD effect in the open field (OF) test after last repeated dose. (A) Time spent in the inner zone was not significantly different during post-dose OF testing. (B) Time in the transition zone was not significantly different during post-dose OF testing. (C). Time spent in the thigmotaxis zone was not significantly different during post-dose OF testing. (D) Velocity was not significantly different during the post dose OF testing. (E) Meandering was not significantly different during post-dose OF testing. (F) High mobility was significantly different between dosing groups but not from control during post-dose OF testing. The * represents a significant difference of P < 0.05. (G). Mobility was not significantly different during post-dose OF testing. (H) Low mobility was not significantly different during post-dose OF testing. Sample sizes for A-H: 0 (n = 54), 1.5 (n = 53), 15 (n = 54), 150 (n = 54). Data presented as mean + /- SD.

Repeated LSD effect in the novel object approach (NOA) test after last repeated dose. (A) Time spent in the inner zone was not significantly different during post-dose NOA testing. (B) Time spent in the transition zone was not significantly different during post-dose NOA testing. (C). Time spent in the thigmotaxis zone was not significantly different during post-dose NOA testing. (D) Velocity was not significantly different during the post dose NOA testing. (E) Meandering was not significantly different during post-dose NOA testing. (F) High velocity was not significantly different during post-dose NOA testing. (G). Mobility was not significantly different during post-dose NOA testing. (H) Low mobility was not significantly different during post-dose NOA testing. Sample sizes for A-H: 0 (n = 54), 1.5 (n = 53), 15 (n = 54), 150 (n = 54). Data presented as mean + /- SD.

Repeated LSD effect in the open field (OF) test during withdrawal. (A) Time spent in the inner zone was not significantly different during withdrawal OF testing. (B) Time spent in the transition zone was not significantly different during withdrawal OF testing. (C). Time spent in the thigmotaxis zone was not significantly different during withdrawal OF testing. (D) Velocity was not significantly different during the withdrawal OF testing. (E) Meandering was not significantly different during post-dose OF testing. (F) High mobility was not significantly different during withdrawal OF testing. (G). Mobility was not significantly different during withdrawal OF testing. (H) Low mobility was not significantly different during withdrawal OF testing. Sample sizes for A-H: 0 (n = 54), 1.5 (n = 52), 15 (n = 54), 150 (n = 54). Data presented as mean + /- SD.

Repeated LSD effect in the novel object approach (NOA) test during withdrawal. (A) Time spent in inner zone was not significantly different during withdrawal NOA testing. (B) Time in transition zone was not significantly different during withdrawal NOA testing. (C). Time spent in thigmotaxis was significantly different between LSD groups during withdrawal NOA testing. The * represents a significant difference of P < 0.05. (D) Velocity was not significantly different during the withdrawal NOA testing. (E) Meandering was not significantly different during withdrawal NOA testing. (F) High velocity was not significantly different during withdrawal NOA testing. (G). Mobility was not significantly different during withdrawal NOA testing. (H) Low mobility was not significantly different during withdrawal NOA testing. Sample sizes for A-H: 0 (n = 54), 1.5 (n = 52), 15 (n = 54), 150 (n = 54). Data presented as mean + /- SD.

Dosing procedure and behavioural testing apparatus. (A) The dosing tanks placed in a white grid closure. (B) Dosing tanks during dosing. (C) Diagram of the dosing procedure. (D) Circular arena used in the OF and NOA tests. Inset is the Lego figurine added prior to the NOA.