Mapping Details

GENETIC MAPPING PANELS

Chr	Location	Mapped As	Panel	Mapped By	Scoring
3	69.1 cM	z3725	Boston MGH Cross (MGH)	Fishman, Mark C.	Data
3	145.0 cM	z3725	Mother of Pearl (MOP)	Postlethwait, John H.	Data
3	467.15 cR	Z3725	Loeb/NIH/5000/4000 (LN54)	Dawid, Igor B.	Data
3	82.0 cM	z3725	Heat Shock (HS)	Woods, Ian G.	Data
3	102.17 cM		Gates et al (GAT)	Talbot, William S.	Data
Note: Physical map location as displayed in the genome browsers is more precise than linkage map location. Note: Physical map location should be used whenever possible.

MAPPING FROM PUBLICATIONS

Marker	Type	Chr	Distance	Publication / Person	Comments
nr5a5	GENE	3		Kuo et al., 2005	Kuo, et al. (2005. Biochem. J. 389:19-26) reports mapping nr5a5 (ff1c) as a  ... Kuo, et al. (2005. Biochem. J. 389:19-26) reports mapping nr5a5 (ff1c) as a SSCP to LG3 at 80.3 cM on the MOP and HS meiotic mapping panels.
dnmt1	GENE	3	16.5 cM	Kupperman et al., 2000	Kupperman et al. (Nature 406, 192-195, 2000) mapped edg5 (mil) 16.5 cM distal  ... Kupperman et al. (Nature 406, 192-195, 2000) mapped edg5 (mil) 16.5 cM distal to Z3725 on LG3. Analysis of 3168 meioses placed edg5 1.48 cM from zae3. edg5 and dnmt1 were co-localized to a 100 kb PAC insert. In addition, no recombination was detected between the two genes in 3168 meioses.
s1pr2	GENE	3		Kupperman et al., 2000	Kupperman et al. (Nature 406, 192-195, 2000) mapped edg5 (mil) 16.5 cM distal  ... Kupperman et al. (Nature 406, 192-195, 2000) mapped edg5 (mil) 16.5 cM distal to Z3725 on LG3. Analysis of 3168 meioses placed edg5 1.48 cM from zae3. edg5 and dnmt1 were co-localized to a 100 kb PAC insert. In addition, no recombination was detected between the two genes in 3168 meioses.
z17222	SSLP	3		Pöpperl et al., 2000	Pöpperl et al. (2000) Mol. Cell 6(2):255-267, report mapping the lazarus, (lzr) locus to LG3 between markers z3725 and z17222, using centromere-linkage analysis. They mapped lzr to a 0.18 cM interval containing the gene pbx4. Sequencing of lzr mutants led to the identification of the lzr locus with the pbx4 gene.
b557	Feature	3		Pöpperl et al., 2000	Pöpperl et al. (2000) Mol. Cell 6(2):255-267, report mapping the lazarus, (lzr) locus to LG3 between markers z3725 and z17222, using centromere-linkage analysis. They mapped lzr to a 0.18 cM interval containing the gene pbx4. Sequencing of lzr mutants led to the identification of the lzr locus with the pbx4 gene.
pbx4	GENE	3		Pöpperl et al., 2000	Pöpperl et al. (2000) Mol. Cell 6(2):255-267, report mapping the lazarus, (lzr) locus to LG3 between markers z3725 and z17222, using centromere-linkage analysis. They mapped lzr to a 0.18 cM interval containing the gene pbx4. Sequencing of lzr mutants led to the identification of the lzr locus with the pbx4 gene.
pbx4	GENE	3	24.0 cR	Vlachakis et al., 2000	Vlachakis eta al. (2000, Dev. Dyn. 217(1):109-119) report mapping pbx4 to LG3 using z3725.

OTHER MAPPING INFORMATION

Chr 3 Pöpperl et al., 2000 Pöpperl et al. (2000) Mol. Cell 6(2):255-267, report mapping the lazarus, (lzr) locus  ... Pöpperl et al. (2000) Mol. Cell 6(2):255-267, report mapping the lazarus, (lzr) locus to LG3 between markers z3725 and z17222, using centromere-linkage analysis. They mapped lzr to a 0.18 cM interval containing the gene pbx4. Sequencing of lzr mutants led to the identification of the lzr locus with the pbx4 gene.   Chr 3 Kuo et al., 2005 Kuo, et al. (2005. Biochem. J. 389:19-26) reports mapping nr5a5 (ff1c) as a SSCP to LG3 at 80.3 cM  ... Kuo, et al. (2005. Biochem. J. 389:19-26) reports mapping nr5a5 (ff1c) as a SSCP to LG3 at 80.3 cM on the MOP and HS meiotic mapping panels.

Primer Sets:

Strain	Bandsize	Restriction Enzyme	Annealing Temperature [C]
IND	248,0,296		60.0
Forward Primer	ACTAAATCGCACTTCAGCAGCG
Reverse Primer	GGTGTCCTCACATCAGCTGCA
AB	256,262,248		60.0
Forward Primer	ACTAAATCGCACTTCAGCAGCG
Reverse Primer	GGTGTCCTCACATCAGCTGCA
TL	242,256,0		60.0
Forward Primer	ACTAAATCGCACTTCAGCAGCG
Reverse Primer	GGTGTCCTCACATCAGCTGCA
TU	248		60.0
Forward Primer	ACTAAATCGCACTTCAGCAGCG
Reverse Primer	GGTGTCCTCACATCAGCTGCA