Mapping Details

GENETIC MAPPING PANELS

Chr	Location	Mapped As	Panel	Mapped By	Scoring
4	61.6 cM	z11657	Boston MGH Cross (MGH)	Fishman, Mark C.	Data
4	88.8 cM	z11657	Mother of Pearl (MOP)	Postlethwait, John H.	Data
4	1809.0 cR	z11657	Goodfellow T51 (T51)	Geisler, Robert	Data
Note: Physical map location as displayed in the genome browsers is more precise than linkage map location. Note: Physical map location should be used whenever possible.

MAPPING FROM PUBLICATIONS

Marker	Type	Chr	Distance	Publication / Person	Comments
z21636	SSLP	4		Huang et al., 2008	Huang, et al. (2008. Development 135(19): 3209-3218.) reports mapping mutant  ... Huang, et al. (2008. Development 135(19): 3209-3218.) reports mapping mutant sq181 (mypt1) to LG4 between markers z7524, z26623 and z21636, z11657 on overlapping BAC clones DKEY-66D18 and DKEY-28J4.
z7524	SSLP	4		Huang et al., 2008	Huang, et al. (2008. Development 135(19): 3209-3218.) reports mapping mutant  ... Huang, et al. (2008. Development 135(19): 3209-3218.) reports mapping mutant sq181 (mypt1) to LG4 between markers z7524, z26623 and z21636, z11657 on overlapping BAC clones DKEY-66D18 and DKEY-28J4.
z26623	SSLP	4		Huang et al., 2008	Huang, et al. (2008. Development 135(19): 3209-3218.) reports mapping mutant  ... Huang, et al. (2008. Development 135(19): 3209-3218.) reports mapping mutant sq181 (mypt1) to LG4 between markers z7524, z26623 and z21636, z11657 on overlapping BAC clones DKEY-66D18 and DKEY-28J4.
sq181	Feature	4		Huang et al., 2008	Huang, et al. (2008. Development 135(19): 3209-3218.) reports mapping mutant  ... Huang, et al. (2008. Development 135(19): 3209-3218.) reports mapping mutant sq181 (mypt1) to LG4 between markers z7524, z26623 and z21636, z11657 on overlapping BAC clones DKEY-66D18 and DKEY-28J4.
ppp1r12a	GENE	4		Huang et al., 2008	Huang, et al. (2008. Development 135(19): 3209-3218.) reports mapping mutant  ... Huang, et al. (2008. Development 135(19): 3209-3218.) reports mapping mutant sq181 (mypt1) to LG4 between markers z7524, z26623 and z21636, z11657 on overlapping BAC clones DKEY-66D18 and DKEY-28J4.
z11566	SSLP	4		Rottbauer et al., 2001	Using bulked segregant analysis and finer genetic mapping, Rottbauer, et al. (2001. Dev Cell 1:265-275.) report mapping cacna1c to LG04 in the ~0.6cM interval between z11657 and z11566. A physical map of this region was generated using YACs and BACs.
cacna1c	GENE	4		Rottbauer et al., 2001	Using bulked segregant analysis and finer genetic mapping, Rottbauer, et al. (2001. Dev Cell 1:265-275.) report mapping cacna1c to LG04 in the ~0.6cM interval between z11657 and z11566. A physical map of this region was generated using YACs and BACs.
b1075	Feature	4		Sheehan-Rooney et al., 2013
z20450	SSLP	4		Sheehan-Rooney et al., 2013
z6977	SSLP	4		Sheehan-Rooney et al., 2013

OTHER MAPPING INFORMATION

Chr 4 Rottbauer et al., 2001 Using bulked segregant analysis and finer genetic mapping, Rottbauer, et al. (2001. Dev Cell  ... Using bulked segregant analysis and finer genetic mapping, Rottbauer, et al. (2001. Dev Cell 1:265-275.) report mapping cacna1c to LG04 in the ~0.6cM interval between z11657 and z11566. A physical map of this region was generated using YACs and BACs.   Chr 4 Huang et al., 2008 Huang, et al. (2008. Development 135(19): 3209-3218.) reports mapping mutant sq181 (mypt1) to LG4  ... Huang, et al. (2008. Development 135(19): 3209-3218.) reports mapping mutant sq181 (mypt1) to LG4 between markers z7524, z26623 and z21636, z11657 on overlapping BAC clones DKEY-66D18 and DKEY-28J4.   Chr 4 Sheehan-Rooney et al., 2013

Primer Sets:

Strain	Bandsize	Restriction Enzyme	Annealing Temperature [C]
AB	178,164,0,158		60.0
Forward Primer	GTGGCCTTTCATTTCAAACG
Reverse Primer	CTGGCGACAATAAAAGGCAT
IND	0,178		60.0
Forward Primer	GTGGCCTTTCATTTCAAACG
Reverse Primer	CTGGCGACAATAAAAGGCAT
EKW	0,162		60.0
Forward Primer	GTGGCCTTTCATTTCAAACG
Reverse Primer	CTGGCGACAATAAAAGGCAT
TU	174,156		60.0
Forward Primer	GTGGCCTTTCATTTCAAACG
Reverse Primer	CTGGCGACAATAAAAGGCAT