PUBLICATION

Mypt1-mediated spatial positioning of Bmp2-producing cells is essential for liver organogenesis

Authors
Huang, H., Ruan, H., Aw, M.Y., Hussain, A., Guo, L., Gao, C., Qian, F., Leung, T., Song, H., Kimelman, D., Wen, Z., and Peng, J.
ID
ZDB-PUB-080915-1
Date
2008
Source
Development (Cambridge, England)   135(19): 3209-3218 (Journal)
Registered Authors
Kimelman, David, Peng, Jinrong, Wen, Zilong
Keywords
Liver organogenesis, Mypt1, Bmp, Zebrafish
MeSH Terms
  • Amino Acid Substitution
  • Animals
  • Animals, Genetically Modified
  • Apoptosis
  • Base Sequence
  • Body Patterning/genetics
  • Body Patterning/physiology
  • Bone Morphogenetic Protein 2/biosynthesis*
  • Bone Morphogenetic Protein 2/genetics
  • Cell Movement
  • Cell Proliferation
  • DNA/genetics
  • Hepatocytes/cytology
  • Hepatocytes/metabolism
  • Liver/cytology
  • Liver/embryology*
  • Liver/metabolism*
  • Mesoderm/embryology
  • Mesoderm/metabolism
  • Mutation
  • Myosin-Light-Chain Phosphatase/chemistry
  • Myosin-Light-Chain Phosphatase/genetics
  • Myosin-Light-Chain Phosphatase/metabolism*
  • Protein Phosphatase 1/genetics
  • Protein Phosphatase 1/metabolism
  • Signal Transduction
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics
PubMed
18776143 Full text @ Development
Abstract
Mesodermal tissues produce various inductive signals essential for morphogenesis of endodermal organs. However, little is known about how the spatial relationship between the mesodermal signal-producing cells and their target endodermal organs is established during morphogenesis. Here, we report that a mutation in the zebrafish myosin phosphatase targeting subunit 1 (mypt1) gene causes abnormal bundling of actin filaments and disorganization of lateral plate mesoderm (LPM) and endoderm cells. As a result, the coordination between mesoderm and endoderm cell movements is disrupted. Consequently, the two stripes of Bmp2a-expressing cells in the LPM fail to align in a V-shaped pocket sandwiching the liver primordium. Mispositioning Bmp2a-producing cells with respect to the liver primordium leads to a reduction in hepatoblast proliferation and final abortion of hepatoblasts by apoptosis, causing the liverless phenotype. Our results demonstrate that Mypt1 mediates coordination between mesoderm and endoderm cell movements in order to carefully position the liver primordium such that it receives a Bmp signal that is essential for liver formation in zebrafish.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes