Transgenic Construct

Tg(top:GFP)

ID

ZDB-TGCONSTRCT-070117-137

Name

Tg(top:GFP)

Previous Names

TOPdGFP (1)
Tg(OTM:GFP)
Tg(TOP:GFP)
tg(top:gfp)

Type

engineered_plasmid

Regulatory Regions

None

Coding Sequences

GFP (1)

Contains Sequences

OTM (1)

Note

The TOPdGFP reporter construct contains four consensus Lef binding sites and a minimal cFos promoter, driving a destabilized GFP transgene. This reporter should only be transcriptionally active in the presence of both stabilized beta-catenin and Lef/Tcf proteins. -- ZDB-PUB-020115-2

Plasmid Map

None

Genomic Features

That Utilize Tg(top:GFP) Construct

Genomic Feature	Affected Genomic Regions
w25Tg

Transgenics

That Utilize Tg(top:GFP) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
apc^hu745; w25Tg	apc		Fig. 4 from Shelton et al., 2006
apc^hu745; w25Tg + MO1-tcf7l2			Fig. 4 from Shelton et al., 2006
apc^zf134/zf134; axin1^tm13/tm13; w25Tg	apc axin1	Fig. 1 from Tee et al., 2009	Fig. 1 from Tee et al., 2009
apc^zf134/zf134; w25Tg	apc	Fig. 1 from Paridaen et al., 2009	2 figures from 2 publications
lef1^zd11/zd11; w25Tg	lef1	Fig. 1 from Xie et al., 2017	Fig. 1 from Xie et al., 2017
med12^y82/y82; w25Tg/+	med12		Fig. 3 from Spikol et al., 2018
sfpq^kg41/kg41; w25Tg	sfpq		Fig. S1 from Thomas-Jinu et al., 2017
w25Tg			31 figures from 28 publications
w25Tg (AB)			Fig. 1 from Veien et al., 2008
w25Tg + MO1-apc			Fig. 1 from Phelps et al., 2009
w25Tg + MO1-ccdc136b		Fig. 4 from Wei et al., 2016	Fig. 4 from Wei et al., 2016
w25Tg + MO1-ctnnb1		Fig. S5 from Upadhyay et al., 2008	Fig. S5 from Upadhyay et al., 2008
w25Tg + MO1-cyp2aa3,cyp2aa9		Fig. 3 from Chen et al., 2016	Fig. 3 from Chen et al., 2016
w25Tg + MO1-dock4a		2 figures from Upadhyay et al., 2008	2 figures from Upadhyay et al., 2008
w25Tg + MO1-dot1l + MO4-tp53		Fig. 7 from Mahmoudi et al., 2010	Fig. 7 from Mahmoudi et al., 2010
w25Tg + MO1-fam53b + MO4-tp53			Fig. 1 from Kizil et al., 2014
w25Tg + MO1-hdac1 + MO1-nlk2 + MO4-tp53			Fig. 7 from Ota et al., 2012
w25Tg + MO1-hdac1 + MO4-tp53			Fig. 7 from Ota et al., 2012
w25Tg + MO1-hmgb1a + MO1-hmgb2a + MO2-hmgb1a + MO2-hmgb2a		Fig. 6 from Itou et al., 2011	2 figures from Itou et al., 2011
w25Tg + MO1-hmgb1a + MO2-hmgb1a		Fig. 6 from Itou et al., 2011	Fig. 6 from Itou et al., 2011
w25Tg + MO1-hmgb2a + MO2-hmgb2a		Fig. 6 from Itou et al., 2011	Fig. 6 from Itou et al., 2011
w25Tg + MO1-lef1		Fig. 3 from Ishitani et al., 2005	Fig. 3 from Ishitani et al., 2005
w25Tg + MO1-mllt10 + MO4-tp53		Fig. 7 from Mahmoudi et al., 2010	Fig. 7 from Mahmoudi et al., 2010
w25Tg + MO1-net1			Fig. 2 from Wei et al., 2017
w25Tg + MO1-nlk2			2 figures from Ota et al., 2012
w25Tg + MO1-nlk2 + MO4-tp53			2 figures from Ota et al., 2012
w25Tg + MO1-nrarpa		Fig. 3 from Ishitani et al., 2005	Fig. 3 from Ishitani et al., 2005
w25Tg + MO1-prr7			Fig. s7 from Kim et al., 2017
w25Tg + MO1-wnt1 + MO4-tp53			Fig. 4 from Ota et al., 2012
w25Tg + MO2-bptf + MO3-bptf			Fig. 6 from Ma et al., 2015
w25Tg + MO2-dot1l + MO4-tp53		Fig. 7 from Mahmoudi et al., 2010	Fig. 7 from Mahmoudi et al., 2010
w25Tg + MO2-fam53b + MO4-tp53			Fig. 1 from Kizil et al., 2014
w25Tg + MO2-lef1			2 figures from Ota et al., 2012
w25Tg + MO2-lef1 + MO4-tp53			Fig. 1 from Ota et al., 2012
w25Tg + MO2-mllt10 + MO4-tp53		Fig. 7 from Mahmoudi et al., 2010	Fig. 7 from Mahmoudi et al., 2010
w25Tg + MO2-nlk2			Fig. 1 from Ota et al., 2012
w25Tg + MO2-nlk2 + MO4-tp53			Fig. 1 from Ota et al., 2012
w25Tg + MO2-sfrp5 + MO3-sfrp1a		Fig. 4 from Holly et al., 2014	Fig. 4 from Holly et al., 2014
w25Tg + MO3-fzd5		Fig. 2 from Liu et al., 2016	Fig. 2 from Liu et al., 2016
w25Tg + MO3-lrp5 + MO4-lrp5		Fig. 3 from Willems et al., 2015	Fig. 3 from Willems et al., 2015
w25Tg + MO3-nipblb		Fig. 3 from Mazzola et al., 2019	Fig. 3 from Mazzola et al., 2019
w25Tg + MO3-tcf7l2 + MO4-tp53		Fig. 7 from Mahmoudi et al., 2010	Fig. 7 from Mahmoudi et al., 2010
w25Tg + MO3-tmem88a + MO4-tmem88a		Fig. 9 from Novikov et al., 2013	Fig. 9 from Novikov et al., 2013
w25Tg + MO4-sox17		Fig. 6 from Chung et al., 2011
w25Tg + MO4-tp53			2 figures from 2 publications
w25Tg/+			3 figures from 2 publications
w25Tg/+ (AB)
w25Tg/+ + MO2-ctnnb2			Fig. 2 from Große et al., 2019
w25Tg/+ + MO3-amer1			Fig. 2 from Große et al., 2019
w25Tg/+ + MO4-tp53			Fig. 2 from Große et al., 2019

Sequence Information

Other Construct Pages

None

Citations